| 荷叶生物碱对胰脂肪酶的抑制作用 |
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| 引用本文： | 朱晓青,吕敬慈,霍世欣,司晓晶.荷叶生物碱对胰脂肪酶的抑制作用[J].上海大学学报(自然科学版),2007,13(1):85-87. |
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| 作者姓名： | 朱晓青 吕敬慈 霍世欣 司晓晶 |
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| 作者单位： | 上海大学理学院,上海,200444 |
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| 摘 要： | 荷叶粗粉经过石油醚去脂后，用95%乙醇提取，初步纯化后得荷叶生物碱粗提物.初步探 讨了荷叶生物碱对胰脂肪酶的抑制机理：抑制剂不直接与酶分子作用，而是间接改 变底物乳化来阻碍底物 酶结合达到抑制作用.通过试验测定了抑制常数： K i=0.2 mg /m L.Dixon图和Lineweaver Burk图的特征表明，该抑制剂对胰脂肪酶的抑制呈非竞争性抑制.
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| 关 键 词： | 荷叶 生物碱 脂肪酶 |
| 文章编号： | 1007-2861（2007）01-0085-03 |
| 收稿时间： | 2006-03-20 |
| 修稿时间： | 2006-03-20 |
Inhibitory Effect on Lipase of Alkaloid Derived from Lotus Leaf |
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| ZHU Xiao-qing,LU Jing-ci,HUO Shi-xin,SI Xiao-jing.Inhibitory Effect on Lipase of Alkaloid Derived from Lotus Leaf[J].Journal of Shanghai University(Natural Science),2007,13(1):85-87. |
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| Authors: | ZHU Xiao-qing LU Jing-ci HUO Shi-xin SI Xiao-jing |
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| Institution: | School of Sciences, Shanghai University, Shanghai 200444, China |
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| Abstract: | Ethanol extract of lotus leaves was first obtained,and the extract was then purified using chloroform.The effects of several factors on inhibitory activity are studied.An inhibitory mechanism of the lotus leaf-derived lipase inhibitor is proposed.This inhibitor does not act directly on the lipase molecule but blocks the enzyme-substrate contact by modifying the emulsion of the lipid substrate.The inhibitory constant(K_i) is 0.2 mg/mL.Based on Dixon plots and Linweaver-Burk plots,the lipase inhibitor demonstrates uncompetitive inhibition for lipase. |
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| Keywords: | lotus leaf alkaloid lipase |
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