| Cloning， expression and characterization of a nucleoside diphosphate kinase (NDPK) gene from tobacco |
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| 引用本文： | Zhou Qiyun,Xie Zongming,Zhang Zhigang,Cao Yangrong,Zhang Jinsong and Chen Shouyi. Cloning， expression and characterization of a nucleoside diphosphate kinase (NDPK) gene from tobacco[J]. 自然科学进展(英文版), 2007, 17(8): 906-912 |
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| 作者姓名： | Zhou Qiyun Xie Zongming Zhang Zhigang Cao Yangrong Zhang Jinsong and Chen Shouyi |
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| 作者单位： | Zhou Qiyun~(1，2**) Xie Zongming~(1**) Zhang Zhigang~1 Cao Yangrong~1 Zhang jinsong~(1***) Chen Shouyi~(1***) (1.National Key Laboratory of Plant Genomics，Institute of Genetics and Developmental Biology，Chinese Academy of Sciences，Beijing100101，China;2.Graduate University of Chinese Academy of Sciences，Beijing 100039，China) |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金 |
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| 摘 要： | Nucleoside diphosphate kinase (NDPK) is a housekeeping enzyme that maintains the intracellular levels of all (d)NTPs used in biosynthesis except ATP.Here we report that a full-length cDNA encoding nucleoside diphosphate kinase (NDPK) was cloned us- ing yeast two-hybrid approach.A tobacco NDPK gene was obtained and designated as NtNDPK1.NtNDPK1 is 704 bp in length and en- codes a putative 16.2 kD protein of 148 amino acids.Phylogenic analysis showed that NtNDPK1 is highly homologous to other plant NDPK genes and identified as type I (NDPK1).RNA-gel blot analysis showed that there was no significant difference of NtNDPK1 ex- pression in roots,stems,leaves and buds.And expression of NtNDPK1 was induced by ABA and PEG and repressed by NaCl,but not significantly affected by Paraquat,wounding and low temperature (4℃) treatments,indicating that NtNDPK1 may play a certain role in response to abiotic stress.In vitro phosphorylation assay demonstrated that NtNDPK1 had autophosphoryLation activity.
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Cloning, expression and characterization of a nucleoside diphosphate kinase (NDPK) gene from tobacco |
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| Zhou Qiyun,Xie Zongming,Zhang Zhigang,Cao Yangrong,Zhang Jinsong and Chen Shouyi. Cloning, expression and characterization of a nucleoside diphosphate kinase (NDPK) gene from tobacco[J]. Progress in Natural Science, 2007, 17(8): 906-912 |
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| Authors: | Zhou Qiyun Xie Zongming Zhang Zhigang Cao Yangrong Zhang Jinsong Chen Shouyi |
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| Abstract: | |
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| Keywords: | abiotic stress autophosphorylation activity NtNDPK1 tobacco yeast two-hybrid |
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