| 24份普通白菜遗传多样性的形态特征和RAPD分析 |
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| 引用本文： | 肖禾,王萌,崔丽洁,开国银,潘静娴.24份普通白菜遗传多样性的形态特征和RAPD分析[J].上海师范大学学报(自然科学版),2015,44(6):585-592. |
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| 作者姓名： | 肖禾 王萌 崔丽洁 开国银 潘静娴 |
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| 作者单位： | 上海师范大学,上海师范大学,上海师范大学,上海师范大学,上海师范大学 |
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| 摘 要： | 利用形态特征与RAPD分析技术,分析了国内不同地区的24份普通白菜(Brassica campestris L.ssp.chinensis(L.)Makino var.communis Tsee et Lee.)种质的遗传多样性.结果表明,13个形态学性状的变异系数为12.4%~69.9%,其中叶柄变异最大,子叶变异最小.24份普通白菜材料在形态学聚类的距离5处聚为4类.利用筛选出的15条RAPD引物从24份普通白菜材料中共扩增出113条带,其中多态性条带83条,多态率73.4%,平均每条引物条带7.53条、多态性条带5.53条.RAPD数据聚类在相似系数0.77处,分为8类,与形态聚类间有重叠和细分.以形态聚类4个类别计算的Nei's基因多样性指标H和Shannon信息指数I,均为第Ⅳ大类第Ⅰ大类第Ⅲ大类第Ⅱ大类,第Ⅳ类的H和I分别为0.2395和0.3523.而不同地区则以江淮地区的Nei's基因多样性H和Shannon信息指数I最高,分别为0.2076和0.3098.这些结果与普通白菜种质资源多样性特征以及地区分布相吻合.
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| 关 键 词： | 普通白菜 形态特征 RAPD分析 聚类分析 |
| 收稿时间： | 2015/12/1 0:00:00 |
Morphological character and RAPD analysis of genetic diversity of different types of Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsee et Lee.pakchoi |
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| XIAO He,WANG Meng,CUI Lijie,KAI Guoyin and PAN Jingxian.Morphological character and RAPD analysis of genetic diversity of different types of Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsee et Lee.pakchoi[J].Journal of Shanghai Normal University(Natural Sciences),2015,44(6):585-592. |
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| Authors: | XIAO He WANG Meng CUI Lijie KAI Guoyin and PAN Jingxian |
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| Institution: | Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University,Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University,Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University,Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University and Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University |
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| Abstract: | |
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| Keywords: | Brassica campestris L ssp chinensis (L ) Makino var communis Tsee et Lee Morphological characters RAPD analysis Clustering analysis |
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