| 不结球白菜子叶离体培养与植株再生的研究 |
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| 引用本文： | 卞莹莹,颜彬,崔丽洁,潘静娴,开国银.不结球白菜子叶离体培养与植株再生的研究[J].上海师范大学学报(自然科学版),2015,44(6):579-584. |
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| 作者姓名： | 卞莹莹 颜彬 崔丽洁 潘静娴 开国银 |
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| 作者单位： | 上海师范大学,上海师范大学,上海师范大学,上海师范大学,上海师范大学 |
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| 摘 要： | 以不结球白菜(Brassica campestris ssp.chinensis Makino)"矮萁苏州青"子叶为外植体,研究了激素浓度和AgNO_3浓度组合对不结球白菜离体培养的影响.结果表明:诱导不定芽形成的最佳培养基为MS+0.3 mg/L NAA+5 mg/L 6-BA;在此基础上添加5 mg/L AgNO_3可显著提高不定芽的诱导频率,芽诱导频率高达31.3%;生根最佳培养基为含有0.3 mg/L NAA的1/2MS培养基.该研究初步建立了"矮萁苏州青"的植株再生体系,为用基因工程手段开展不结球白菜分子育种奠定了基础.
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| 关 键 词： | 不结球白菜 植株再生 组织培养 |
| 收稿时间： | 2015/10/1 0:00:00 |
Study on plantlet regeneration and in vitro culture of non-heading Chinese cabbage |
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| BIAN Yingying,YAN Bin,CUI Lijie,PAN Jingxian and KAI Guoyin.Study on plantlet regeneration and in vitro culture of non-heading Chinese cabbage[J].Journal of Shanghai Normal University(Natural Sciences),2015,44(6):579-584. |
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| Authors: | BIAN Yingying YAN Bin CUI Lijie PAN Jingxian and KAI Guoyin |
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| Institution: | Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University,Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University,Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University,Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University and Development Center of Plant Germplasm Resources,College of Life and Environmental Sciences,Shanghai Normal University |
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| Abstract: | |
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| Keywords: | Brassica campestris ssp chinensis Makino plantlet regeneration tissue culture |
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