| Interaction between PII and NifA in Azospirillum brasilense Sp7 |
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| 作者姓名： | CHEN Sanfeng DU Jinping WU Lixian ZHAO Yinsuo & LI Jilun . |
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| 作者单位： | Chen Sanfeng,WU Lixian,Li Jilun(Department of Microbiology, China Agricultural University, Beijing 100094, China);DU Jinping,ZHAO Yinsuo(National Key Lab. for Agrobiotechnology, China Agricultural University, Beijing 100094, China) |
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| 基金项目： | This work was supported by the National 73Project (Grant No. 001CB108904) and the National Natural Science Foundation of China (Grant No.30070407). |
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| 摘 要： |
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Interaction between PII and NifA in Azospirillum brasilense Sp7 |
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| CHEN Sanfeng,DU Jinping,WU Lixian,ZHAO Yinsuo & LI Jilun ..Interaction between PII and NifA in Azospirillum brasilense Sp7[J].Chinese Science Bulletin,2003,48(2). |
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| Authors: | Chen Sanfeng DU Jinping WU Lixian ZHAO Yinsuo Li Jilun |
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| Institution: | e-mail: chensf@ mail.cau.edu.cn |
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| Abstract: | The interaction between PII and NifA in A. brasilense Sp7 was investigated by using the yeast two-hybrid system. Our experimental results showed that PII directly interacted with the entire NifA protein and its N-terminal domain,but did not interact with the central domain and the C-terminal domain of NifA. No interaction happened if glnB coding for PII was frame-shift mutated. Pz, a homolog of PII, had no interation with NifA. |
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| Keywords: | Azospirillum brasilense yeast two-hybrid system PII NifA |
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