| A/PR8/34(H1N1)NA基因包装信号对H5N1流感疫苗株NIBRG-14产量的影响 |
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| 引用本文： | 郑薇,潘蔚绮,杨化强,李婷,张北武,李锋,陈凌. A/PR8/34(H1N1)NA基因包装信号对H5N1流感疫苗株NIBRG-14产量的影响[J]. 中国科学技术大学学报, 2010, 40(4). DOI: 10.3969/j.issn.0253-2778.2010.04.001 |
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| 作者姓名： | 郑薇 潘蔚绮 杨化强 李婷 张北武 李锋 陈凌 |
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| 作者单位： | 1. 中国科学技术大学生命科学学院,安徽合肥,230026;中国科学院广州生物医药与健康研究院,广州,510663;呼吸病国家重点实验室,广州,510120
2. 中国科学技术大学生命科学学院,安徽合肥,230026;呼吸病国家重点实验室,广州,510120 |
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| 基金项目： | 广州市科技计划,中国科学院广州生物医药与健康研究院院内启动经费资助 |
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| 摘 要： | NIBRG-14是采用6+2策略制备的一株H5N1灭活疫苗株,其表面抗原HA和NA基因来自于A/Vietnam/1194/2004(H5N1,VN1194),内部基因来自于A/Puerto Rico/8/34(H1N1,PR8),已有研究表明该疫苗株在鸡胚中的产量不佳.本研究发现,在PR8背景下,VN1194NA基因被包装入重组病毒中的效率仅为正常包装量的38%～68%,因此有一部分重组病毒为不含有NAvRNA的缺陷型病毒粒子.本研究通过在VN1194NA基因完整编码区(CDS)的5′和3′两端嵌合PR8NA基因包装信号序列(vRNA3′末端41bp,5′末端67bp)的方法,使重组病毒中NAvRNA的包装效率得到完全恢复,并且病毒在鸡胚的生长滴度提高了10倍,血凝素HA含量提高了约2·7倍,从而为H5N1流感疫苗株的研制提供了新的思考方向.
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| 关 键 词： | H5N1流感 疫苗 包装信号 病毒生长 |
Enhanced growth of the H5N1 influenza vaccine strain NIBRG-14 by the neuraminidase's packaging signal sequence of A/Puerto Rico/8/34 |
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| ZHENG Wei,PAN Weiqi,YANG Huaqiang,LI Ting,ZHANG Beiwu,LI Feng,CHEN Ling. Enhanced growth of the H5N1 influenza vaccine strain NIBRG-14 by the neuraminidase's packaging signal sequence of A/Puerto Rico/8/34[J]. Journal of University of Science and Technology of China, 2010, 40(4). DOI: 10.3969/j.issn.0253-2778.2010.04.001 |
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| Authors: | ZHENG Wei PAN Weiqi YANG Huaqiang LI Ting ZHANG Beiwu LI Feng CHEN Ling |
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| Affiliation: | ZHENG Wei1,2,3,PAN Weiqi1,YANG Huaqiang1,LI Ting1,ZHANG Beiwu1,LI Feng1,CHEN Ling1,3(1.School of Life Science,University of Science , Technology of China,Hefei 230026,China,2.Guangzhou Institute of Biomedicine , Health,Chinese Academy of Sciences(GIBH),Guangzhou 510663,3.National Key Laboratory of Respiratory Disease,Guangzhou 510120,China) |
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| Abstract: | NIBRG-14 is one of the H5N1 candidate vaccine viruses developed using 6+2 approach with the hemagglutinin(HA) and neuraminidase(NA) genes derived from A/Vietnam/1194/2004(H5N1,VN1194) and the remaining six internal segments from A/Puerto Rico/8/34(H1N1,PR8).However,NIBRG-14 was reported to yield low amounts of HA antigen.This study found that the NA vRNA of VN1194 is poorly packaged(38%~68%) into the recombinant viruses with a backbone of PR8 genes,causing the formation of defective virions without the NA v... |
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| Keywords: | H5N1 influenza vaccine packaging signal virus growth |
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