| Seasonal variation of gut Cyanophyta contents and liver GST expression of mud carp （Cirrhina molitorella） and Nile tilapia （Oreochromis niloticus） in the tropical Xiangang Reservoir （Huizhou, China） |
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| 作者姓名： | CHENG WeiXuan LIANG XuFang SHEN Dan ZHOU Qiong HE Yan HE Shan LI GuangYu |
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| 作者单位： | College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China |
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| 摘 要： | Liver glutathione S-transferase(GST) plays a major role in the detoxification of microcystins(MCs) via conjugation to glutathione(GSH).We evaluated the relationship between seasonal variation in fish gut contents and the expression of GST isoforms in mud carp(Cirrhina molitorella) and Nile tilapia(Oreochromis niloticus).We quantified the abundance and diversity of plankton in the water column and foregut of mud carp and Nile tilapia in the tropical Xiangang Reservoir between October 2007 and July 2008.The mRNA expression of 7 liver GST isoforms was determined by real-time RT-PCR.The gut contents of both species were dependent on the amount and type of phytoplankton and zooplankton in the water.The expression of liver GST genes in Nile tilapia and mud carp was positively and negatively correlated,respectively,with the abundance of toxic cyanobacteria in the fore-gut.The expression of liver GST mRNA was correlated to the abundance of toxic cyanobacteria in the gut contents of both species,suggesting that mRNA expression of GST isoforms could be used as a biomarker in Nile tilapia and mud carp to monitor cyanobacteria blooms in reservoirs.
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| 关 键 词： | glutathione S-transferase seasonal variation Cirrhina molitorella Oreochromis niloticus fish gut Cyanophyta content |
| 收稿时间： | 9 August 2009 |
Seasonal variation of gut Cyanophyta contents and liver GST expression of mud carp (<Emphasis Type="Italic">Cirrhina molitorella</Emphasis>) and Nile tilapia (<Emphasis Type="Italic">Oreochromis niloticus</Emphasis>) in the tropical Xiangang Reservoir (Huizhou,China) |
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| CHENG WeiXuan,LIANG XuFang,SHEN Dan,ZHOU Qiong,HE Yan,HE Shan,LI GuangYu.Seasonal variation of gut Cyanophyta contents and liver GST expression of mud carp (Cirrhina molitorella) and Nile tilapia (Oreochromis niloticus) in the tropical Xiangang Reservoir (Huizhou,China)[J].Chinese Science Bulletin,2012,57(6):615-622. |
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| Authors: | WeiXuan Cheng XuFang Liang Dan Shen Qiong Zhou Yan He Shan He GuangYu Li |
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| Institution: | (1) Pearl River Fishery Research Institute, Chinese Academy of Fishery Science, Guangzhou, 510380, China;(2) University Toulouse, UMR 5174, EDB, CNRS-Univ. Paul Sabatier, 31062 Cedex Toulouse, France;; |
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| Abstract: | Liver glutathione S-transferase (GST) plays a major role in the detoxification of microcystins (MCs) via conjugation to glutathione
(GSH). We evaluated the relationship between seasonal variation in fish gut contents and the expression of GST isoforms in
mud carp (Cirrhina molitorella) and Nile tilapia (Oreochromis niloticus). We quantified the abundance and diversity of plankton in the water column and foregut of mud carp and Nile tilapia in the
tropical Xiangang Reservoir between October 2007 and July 2008. The mRNA expression of 7 liver GST isoforms was determined
by real-time RT-PCR. The gut contents of both species were dependent on the amount and type of phytoplankton and zooplankton
in the water. The expression of liver GST genes in Nile tilapia and mud carp was positively and negatively correlated, respectively,
with the abundance of toxic cyanobacteria in the fore-gut. The expression of liver GST mRNA was correlated to the abundance
of toxic cyanobacteria in the gut contents of both species, suggesting that mRNA expression of GST isoforms could be used
as a biomarker in Nile tilapia and mud carp to monitor cyanobacteria blooms in reservoirs. |
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| Keywords: | glutathione S-transferase seasonal variation Cirrhina molitorella Oreochromis niloticus fish gut Cyanophyta content |
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