| 硅胶G柱层析法制备卵黄卵磷脂工艺的研究 |
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| 引用本文： | 臧荣鑫,杨具田,蔡勇,徐红伟. 硅胶G柱层析法制备卵黄卵磷脂工艺的研究[J]. 西北民族学院学报, 2009, 30(3): 42-44,47 |
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| 作者姓名： | 臧荣鑫 杨具田 蔡勇 徐红伟 |
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| 作者单位： | 臧荣鑫,杨具田,徐红伟(西北民族大学,生命科学与工程学院,甘肃,兰州,730030);蔡勇(西北民族大学,实验中心,甘肃,兰州,730030) |
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| 摘 要： | 以鸡蛋卵黄为材料,采用二元有机溶剂为提取剂,丙酮为沉淀剂,应用正交试验确定卵黄卵磷脂的最佳提取条件;选择硅胶G为吸附剂,以不同有机溶剂配比组成的洗脱体系为洗脱剂,经单因素试验柱层析法纯化卵磷脂.结果表明:提取温度25℃,提取时间60min,醇醚体积比4∶1提取效果最佳;无水乙醇、甲醇混合溶剂为最佳纯化洗脱剂组合,并获得了纯度达99.3%的卵黄卵磷脂.
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| 关 键 词： | 蛋黄卵磷脂 硅胶G柱层析 分离纯化 |
Technique Study of Purifying Yolk Lecithin with Column Chromatography |
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| Affiliation: | ZANG Rong - xin, YANG Ju - tian, CAI Yong, FENG Yu - lan, XU Hong - wei (Life Science and Engineering College of Northwest University for Nationalities, Lanzhou Gansu, 730030, China) |
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| Abstract: | The yolk was used to extract lecithin. Silica- gel was used as absorbent and the orthogonal test was used to confirm the best condition. 99.3% purity of the purified lecithin was obtained by column chromatography purification. The results showed the optimal conditions were temperature 25℃, time 60 min, the ratio of alcohol ether 4 ： 1 respectively. The mixture solution of alcohol and methanol were the best solvent. |
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| Keywords: | yolk lecithin chromatography purification |
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