甲状腺乳头状癌RASSF1A启动子异常甲基化的检测

为了研究甲状腺乳头状癌组织中RASSF1A基因启动子区甲基化状态,探讨其甲基化与临床参数之间的关系,运用甲基化特异性PCR(MSP)技术,对51例甲状腺乳头状癌组织及10例正常甲状腺组织中RASSF1A基因的甲基化状况进行分析。结果表明:甲状腺乳头状癌组织中RASSF1A基因甲基化率为68.6%(35/51),而正常甲状腺组织中未检测到该基因的甲基化,两者之间的差异有统计学意义(P<0.05);RASSF1A基因甲基化与患者年龄、性别无相关性与肿瘤的浸润和转移有相关性,差异有统计学意义(P<0.05)。因此,RASSF1A基因甲基化是甲状腺乳头状癌组织中常见的分子生物学事件,在腺癌的发生发展过程中发挥重要作用。

The Methylation of RASSF1A Gene in Papillary Thyroid Carcinoma

To observe the methylation status of RASSF1A gene in papillary thyroid carcinoma and its correlation with clinical paraments.Methods: Methylation-specific PCR(MSP) was used to analyze the methylation status of RASSF1A gene in 51 papillary thyroid carcinomas and 10 normal thyroid tissues.Results:Hypermethylation of RASSF1A gene was observed in 68.6%(35/51) of PTC(pap illary thyroid carcinoma).None of the normal thyroid tissues was methylated,compared to the carcinoma,P<0.05.There was no evidence to demonstrate the relationship between the methylation of RASSF1A gene and the patients'age and gender(P>0.05).The methylation of RASSF1A gene was positively related with metastasis(P<0.05).Conclusion:The methylation of RASSF1A gene is one of the important molecular biological events and is related to metastasis,which may be involved in thyroid tumorigenesis.