| Mir—92a在癫痫中的表达及意义 |
| |
| 引用本文： | 杨小兰,王衡,陈阳美. Mir—92a在癫痫中的表达及意义[J]. 世界科技研究与发展, 2012, 0(2): 287-289,340 |
| |
| 作者姓名： | 杨小兰 王衡 陈阳美 |
| |
| 作者单位： | 重庆医科大学附属第二医院神经内科,重庆400010 |
| |
| 基金项目： | 重庆市自然科学基金计划项目任务书（CSTC2010BB5106）,重庆市卫生局2010年度医学科研计划项目（重点项目）（2010-1-36）资助 |
| |
| 摘 要： | 目的检测癫痫中 mir 92a表达水平,以探索 mir 92a表达与癫痫发生发展之间的关系.方法应用原位杂交技术检测氯化锂-匹罗卡品大鼠癫痫模型发作后6h、72h、14d和60d脑组织中 mir 92a的表达水平;应用荧光定量 PCR技术在氯化锂-匹罗卡品大鼠癫痫模型各时间点和在难治性癫痫患者脑组织中检测 mir 92a的表达情况.结果在氯化锂-匹罗卡品大鼠癫痫模型的6h、72h(P<0.05)中 mir 92a表达水平相比正常对照下降,而在14d和60d(P<0.05)中 mir 92a表达升高;在难治性癫痫患者脑组织中 mir 92a的表达比非癫痫病人升高(P<0.05).结论 mir 92a在癫痫模型和难治性癫痫患者脑组织中存在异常的表达,可能通过调节钾离子-氯离子联合转运体2表达进而参与了癫痫的发生发展过程
|
| 关 键 词： | 癫痫 microRNA 钾离子-氯离子联合转运体 2 |
Expression and Meaning of Mir-92a in Epilepsy |
| |
| YANG Xiaolan,WANG Heng,CHEN Yangmei. Expression and Meaning of Mir-92a in Epilepsy[J]. World Sci-tech R & D, 2012, 0(2): 287-289,340 |
| |
| Authors: | YANG Xiaolan WANG Heng CHEN Yangmei |
| |
| Affiliation: | (Department of Neurology ,The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010) |
| |
| Abstract: | Objective To investigate mir-92a expression in epilepsy in order to explore the possible roles of mir-92a in the pathogenesis of epilepsy. Methods Mir-92a expression was detected in the rat epileptic model of Lithium-piloearpine and in patients with drug-refractory epilepsy using real-time PCR and in situ hybridization. Results Comparing with control rats mir-92a expression was down-regulated at time points of 6 h and 72 h （P 〈 0. 05 ）in the rat epileptic model of Lithium-pilocarpine, but was up-regulated at time points of 14d and 60d （P 〈 0. 05 ）. In patients with drug-refractory epilepsy mir-92a expression was higher than the control group （ P 〈 0. 05 ）. Condution Mir-92a is likely to involve in the pathogenesis of epilepsy through mediating potassium-chloride cotransporter2 （KCC2） expression. |
| |
| Keywords: | epilepsy mieroRNA potassium-chloride cotransporter2 |
| 本文献已被 维普 万方数据 等数据库收录！ |
