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| 人骨形成蛋白-4和人骨形成蛋白-7在毕赤酵母中的共表达 | |
| 引用本文： | 陈雅娟,黄义德,张彦定.人骨形成蛋白-4和人骨形成蛋白-7在毕赤酵母中的共表达[J].福建师范大学学报(自然科学版),2007,23(1):78-82,108. |
| 作者姓名： | 陈雅娟 黄义德 张彦定 |
| 作者单位： | 1. 福建师范大学生命科学学院,福建,福州,350108 2. 福建师范大学生命科学学院,福建,福州,350108;发育与神经生物学福建省高校重点实验室,福建,福州,350108 3. 福建师范大学生命科学学院,福建,福州,350108;发育与神经生物学福建省高校重点实验室,福建,福州,350108;农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350108 |
| 基金项目： | 福建省发展改革委员会基金 , 福建省自然科学基金 , 福建省教育厅科研项目 |
| 摘 要： | 利用人骨形成蛋白-4(hBMP4)与人骨形成蛋白-7(hBMP7)的成熟肽cDNA片段制备转基因毕赤酵母菌株,实现了hBMP4与hBMP7在巴斯德毕赤酵母细胞中的共表达.Western-blotting分析表明,表达产物含有hBMP4与hBMP7,片段大小分别为26 kD和17 kD,均为单体蛋白. |
| 关 键 词： | 人骨形成蛋白-4 人骨形成蛋白-7 毕赤酵母 共表达 |
| 文章编号： | 1000-5277（2007）01-0078-05 |
| 修稿时间： | 2006-05-19 |
Co-expression of hBMP4 and hBMP7 in Pichia pastoris |
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| CHEN Ya-juan,HUANG Yi-de,ZHANG Yan-ding.Co-expression of hBMP4 and hBMP7 in Pichia pastoris[J].Journal of Fujian Teachers University(Natural Science),2007,23(1):78-82,108. | |
| Authors: | CHEN Ya-juan HUANG Yi-de ZHANG Yan-ding |
| Institution: | 1. College of Life Sciences, Fujian Normal University, Fuzhou 350108, China 2. State Key Laboratory of Developmental Biology and Neurobiology, Institution for Higher Learning in Fujian, Fuzhou 350108, China; 3. Key Lab of Eco-physiology Genetics Improvement of Sugarcane, Ministry of Agriculture, Fuzhou 350108, China |
| Abstract: | The techniques for co-expression of the mature domain of hBMP4 and hBMP7 in Pichia pastorls were established. The cDNA mature domains that encodes BMP4 and BMP7 were cloned into pPICgK and the resulting constructs were transformed into Pichia pastoris for co-expression. Western-blotting analysis by anti-BMP4 antibody and anti-BMP7 antibody demonstrated that hBMP4 and hBMP7 were co-expressed in culture supernatant. Further analysis showed that the expressed hBMP4 and hBMP7 were monomers, with the molecular weight of 26 000 and 17 000 dalton, respectively. |
| Keywords: | hBMP4 hBMP7 Pichia pastoris co-expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |