| 钙黄绿素荧光猝灭法测定辛硫磷 |
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| 引用本文： | 程定玺,杜钢锋,毕新慧,盛国英,傅家谟. 钙黄绿素荧光猝灭法测定辛硫磷[J]. 河南师范大学学报(自然科学版), 2010, 38(4) |
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| 作者姓名： | 程定玺 杜钢锋 毕新慧 盛国英 傅家谟 |
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| 作者单位： | 中科院广州地球化学研究所,有机地球化学重点实验室,广州,510640;河南师范大学,化学与环境科学学院,河南,新乡,453007;河南师范大学,化学与环境科学学院,河南,新乡,453007;中科院广州地球化学研究所,有机地球化学重点实验室,广州,510640 |
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| 摘 要： | 中性介质中,生物小分子钙黄绿素与阳离子表面活性剂CTMAB缔合,体系荧光强度降低,而当加入辛硫磷后荧光强度继续降低并且降低的程度与加入辛硫磷的量在一定浓度范围内成线性.据此建立了一种测定辛硫磷的新方法,该法的线性范围为0～5.6mg/L,检测限为0.039mg/L.
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| 关 键 词： | 钙黄绿素 溴化十六烷基三甲基铵 辛硫磷 |
Fluorescence Quenching Method for the Determination of Phoxim by Calcein |
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| CHENG Ding-xi,DU Gang-feng,BI Xin-hui,SHENG Guo-ying,FU Jia-mo. Fluorescence Quenching Method for the Determination of Phoxim by Calcein[J]. Journal of Henan Normal University(Natural Science), 2010, 38(4) |
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| Authors: | CHENG Ding-xi DU Gang-feng BI Xin-hui SHENG Guo-ying FU Jia-mo |
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| Abstract: | In the presence of appropriate amounts of cationic surfactant of cetyl trimethyl ammonium bromide(CTMAB),the anionic dye calcein combind with it and the fluorescence intensity of calcein-CTMAB can be decreased obviously by adding phoxim in the neutral medium.The decreased fluorescence intensity is proportional to the concentration of phoxim in rang of 0-5.6 mg/L and the detection limit is 0.039 mg/L. |
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| Keywords: | calcein cetyl trimethyl ammonium bromide phoxim |
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