| 磺胺胍抗性筛选法选育L-色氨酸高产菌株及其发酵条件的优化 |
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| 引用本文： | 李阳,刘峰,卢龙娣.磺胺胍抗性筛选法选育L-色氨酸高产菌株及其发酵条件的优化[J].福建师范大学学报(自然科学版),2009,25(3). |
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| 作者姓名： | 李阳 刘峰 卢龙娣 |
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| 作者单位： | 福建师范大学生命科学学院,福建,福州,350108 |
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| 摘 要： | 以谷氨酸棒杆菌FC22(Phe-+Tyr-+5MTr)为出发菌株,经过紫外线诱变,定向选育出具有磺胺胍抗性目的突变株FC523(Phe-+Tyr-+5MTr+SGr).通过正交实验对FC523的发酵培养基进行优化,并通过单因素实验对温度、pH和转速等发酵条件进行研究.在最佳条件下,该菌株摇瓶积累L-色氨酸最高可达8.6 g/L.
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| 关 键 词： | L-色氨酸 诱变育种 磺胺胍 抗性筛选 发酵 |
Screening of Mutant Overproducing L-tryptophan with Sulfonamide Resistance Method and Optimization of the Fermentation Conditions |
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| LI Yang,LIU Feng,LU Long-di.Screening of Mutant Overproducing L-tryptophan with Sulfonamide Resistance Method and Optimization of the Fermentation Conditions[J].Journal of Fujian Teachers University(Natural Science),2009,25(3). |
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| Authors: | LI Yang LIU Feng LU Long-di |
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| Institution: | College of Life Sciences;Fujian Normal University;Fuzhou 350108;China |
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| Abstract: | A sulfonamide resistant mutant FC523(Phe-+Tyr-+5MTr+SGr)was obtained from Corynebacterium glutamicum FC22(Phe-+Tyr-+5MTr)by UV treatment.The culture medium was optimized by orthogonal design experiment and fermentation conditions were studied by single factor experiment.Tryptophan was accumulated to 8.6 g/L by strain FC523 at the optimized conditions. |
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| Keywords: | L-tryptophan mutation breeding sulfonamide resistance screen fermentation |
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