| 金黄色葡萄球菌核酸酶及其突变体酶动力学性质的比较 |
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| 引用本文： | 蔡勤,徐晋麟.金黄色葡萄球菌核酸酶及其突变体酶动力学性质的比较[J].上海交通大学学报,1997,31(4):93-97. |
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| 作者姓名： | 蔡勤 徐晋麟 |
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| 作者单位： | 中科院上海生物工程研究中心 |
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| 摘 要： | 将金黄色葡萄球菌核酸酶的类似物及其不同点突变体的基因进行表达，经Ｂｉｏ－Ｒｅｘ７０柱层析及尿处理进行分离纯化，在ＳＤＳ－ＰＡＧＥ上显示一条蛋白带，相对分子质量约为１７ｋＤ。测试了它们的比活力，Ｋｍ，Ｖｍａｘ，Ｋｉ等酶动力学参数。结果表明，ＳＮａｓｅＲ与ＳＮａｓｅ的催化活性无显著差异，但ＳＮａｓｅＲ对底物的亲和力明显增加。
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| 关 键 词： | 金黄色葡萄球菌 核酸酶 突变体 酶动力学 |
Comparative Studies of the Enzymological Characteristics of SNase and Its Mutants |
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| Cai Qin,Xu Jinlin.Comparative Studies of the Enzymological Characteristics of SNase and Its Mutants[J].Journal of Shanghai Jiaotong University,1997,31(4):93-97. |
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| Authors: | Cai Qin Xu Jinlin |
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| Institution: | Department of Biological Science and Technology |
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| Abstract: | Staphylococcal nuclease (SNase),some of its truncation mutants (SNase R) and site directed mutants are over produced by recombinant DNA manipulation and purified by Bio Rex 70 column chromatography.The results are shown on SDS PAGE.The comparative studies of enzymatic kinetics characters of SNase,SNase R and three site directed mutants show that adding six N terminated amino acid residues to SNase may influence the enzymatic kinetics in a slight level,three site directed mutants have stronger affinity for the substrate (DNA or pdTp),in which SNase R 113 is the strongest. |
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| Keywords: | staphylococcal nuclease mutant enzymatic kinetics |
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