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流动注射化学发光法测定阿卡波糖
引用本文:赵冕,王贝贝,王冰,李献锐. 流动注射化学发光法测定阿卡波糖[J]. 河北大学学报(自然科学版), 2022, 42(3): 251. DOI: 10.3969/j.issn.1000-1565.2022.03.005
作者姓名:赵冕  王贝贝  王冰  李献锐
作者单位:河北医科大学教学实验中心,河北石家庄 050017;河北医科大学药学院,河北石家庄 050017
基金项目:河北省自然科学基金资助项目(B2020206009);河北省高等学校科学技术研究项目(QN2021101)
摘    要:建立一种流动注射化学发光法测定阿卡波糖的新方法.在碱性条件下,阿卡波糖对H2O2-鲁米诺发光体系的发光强度具有显著的增敏作用,在最佳实验条件下,发光强度的增加值(ΔI)与阿卡波糖的质量浓度在3.0×10-4~2.0×10-2 mg/mL内成线性关系,相关系数为0.998 7.方法的检出限为2.3×10-4 mg/mL,对0.01 mg/mL的阿卡波糖连续测定11次,其相对标准偏差为2.7%,并应用于阿卡波糖片剂含量的测定,平均回收率为101.6%.本方法操作简单快速,重现性好,可用于阿卡波糖的含量测定.

关 键 词:阿卡波糖  流动注射  化学发光  增敏
收稿时间:2021-10-20

Flow injection chemiluminescence method for determination of acarbose
ZHAO Mian,WANG Beibei,WANG Bing,LI Xianrui. Flow injection chemiluminescence method for determination of acarbose[J]. Journal of Hebei University (Natural Science Edition), 2022, 42(3): 251. DOI: 10.3969/j.issn.1000-1565.2022.03.005
Authors:ZHAO Mian  WANG Beibei  WANG Bing  LI Xianrui
Affiliation:1.Experimental Center for Teaching, Hebei Medical University, Shijiazhuang 050017, China; 2.College of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China
Abstract:A simple and rapid flow-injection chemiluminescence(FI-CL)method was developed for the determination of acarbose in tablets based on the fact that acarbose can remarkably enhance the chemiluminescence of Luminol-H2O2 in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence relative intensity had a good linear relationship with acarbose concentration in the range of 3×10-4~2×10-2 mg/mL with a correlation coefficient of 0.998 7. The detection limit was 2.3×10-4 mg/mL and RSD(n=11)is 2.7% for 0.01 mg/mL acarbose. The proposed method has been successfully applied for the determination of acarbose in tablets sample and the average recovery was 101.6%.
Keywords:acarbose  flow injection  chemiluminescence  sensitization  
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