| 富硒鸡腿菇菌丝体深层发酵培养基的优化 |
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| 引用本文： | 余杰,崔鹏举,崔仕超,赖晓,李值.富硒鸡腿菇菌丝体深层发酵培养基的优化[J].汕头大学学报(自然科学版),2008,23(3):45-50. |
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| 作者姓名： | 余杰 崔鹏举 崔仕超 赖晓 李值 |
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| 作者单位： | 汕头大学生物学系,广东,汕头,515063 |
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| 摘 要： | 研究营养因子对鸡腿菇菌丝体生长和富硒的影响,筛选到适合鸡腿菇富硒深层发酵的优化培养基配方为:葡萄糖2%,玉米粉4%,花生粕0.4%,KH2PO4 0.2%,MgSO4·7H2O0.1%,VB1 10×10-3 g/L,Na2SeO3 4.4×10-3 g/L.在此优化培养基的基础上进行5 d的摇瓶发酵培养,得到菌丝体生物量为18.14 g/L,富硒率为56.35%.
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| 关 键 词： | 鸡腿菇 深层培养 富硒 |
Optimization of Fermentation Liquid Medium for the Se-Accumulation of Coprinus comatus |
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| YU Jie,CUI Peng-ju,CUI Shi-chao,LAI Xiao,LI Zhi.Optimization of Fermentation Liquid Medium for the Se-Accumulation of Coprinus comatus[J].Journal of Shantou University(Natural Science Edition),2008,23(3):45-50. |
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| Authors: | YU Jie CUI Peng-ju CUI Shi-chao LAI Xiao LI Zhi |
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| Institution: | (Department of Biology, Shantou University, Shantou 515063, Guangdong, China) |
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| Abstract: | The effects of the nutrition factor on the biomass and Se-accumulation of Coprinus comatus mycelia were studied. An optimum medium was selected through orthogonal test. The medium composition was as follows： 2.0% glucose, 4% maize power, 0.4% peanut meal, 0.2% KH2PO4, 0.1% MgSO4·7H2O, 10×10^-3g/L VB1, 4.4× 10^-3 g/L Na2SeO3, The results showed that under these conditions, the production of mycelia biomass and the ratio of Se-accumulation was 18.14g/L and 56.35% respectively, when the mycelia had been cultured about 5 d in the shaking flask. |
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| Keywords: | Coprinus comatus Submerged culture Se-accumulation |
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