Method of transforming resistance gene to carbendazim intoTrichoderma sp.

The method of transforming resistance gene to carbendazim intoTrichoderma sp. was studied by using genetic engineering technique. The results show that the time of fungicide selection on the transformedTrichoderma sp. may have very important effect on the resistance gene transformation. By this method, the resistance gene to carbendazim was transformed intoTrichoderma harzianum, a strain ofT. harzianum with resistance to carbendazim was achieved. The transformant could grow on the medium containing 150 μg/mL carbendazim. The resistance is stable after 10 times transfer on non-selective medium.