| 薄层分离-分光光度法测定夹竹桃枝中三萜类化合物的质量分数 |
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| 引用本文： | 孟繁旭,白丽明,马玲,安东政义. 薄层分离-分光光度法测定夹竹桃枝中三萜类化合物的质量分数[J]. 高师理科学刊, 2011, 31(6): 59-61. DOI: 10.3969/j.issn.1007-9831.2011.06.020 |
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| 作者姓名： | 孟繁旭 白丽明 马玲 安东政义 |
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| 作者单位： | 1. 齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔,161006
2. 日本新当(淀)大学,日本新(淀)950-2181 |
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| 基金项目： | 教育部留学回国人员科研启动基金(2009-36-1341); 黑龙江省留学归国科学基金(LC201008) |
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| 摘 要： | 为测定夹竹桃中总三萜的质量分数,以齐墩果酸为对照品,5％的香草醛-冰醋酸溶液和高氯酸为显色剂,采用薄层色谱分离-分光光度法在550 nm波长处测定样品吸光度.用正交试验法优化了显色反应条件,适宜的展开剂组成为氯仿∶丙酮=10∶3,对照品在10～200μL/mL范围呈良好线性关系,其回归方程为Y=0.007 0C+0.0...
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| 关 键 词： | 夹竹桃 三萜类化合物 分光光度法 薄层色谱 |
Quantitative analysis of triterpenoids from the bark of Nerium oleander L by thin layer chromatography-spectrophotometry |
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| MENG Fan-xu,BAI Li-ming,MA Ling,ANDO Masayoshi. Quantitative analysis of triterpenoids from the bark of Nerium oleander L by thin layer chromatography-spectrophotometry[J]. Journal of Science of Teachers＇College and University, 2011, 31(6): 59-61. DOI: 10.3969/j.issn.1007-9831.2011.06.020 |
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| Authors: | MENG Fan-xu BAI Li-ming MA Ling ANDO Masayoshi |
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| Affiliation: | MENG Fan-xu1,BAI Li-ming1,MA Ling1,ANDO Masayoshi2(1.Chemistry and Chemical Engineering Institute,Qiqihar University,Qiqihar 161006,China,2.Niigata University,Ikarashi 2-8050,Niigata 950-2181,Japan) |
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| Abstract: | Objective to determination the triterpenoids content in the bark of Nerium oleander L. Under the experimental conditions including oleanolie acid as the reference. 5% vanillin-acetie acid and perehloric acid as color developers, the content of triterp-enoids in Nerium oleander L was determined by the thin laver ehromatographv-spectrophotometric method at 550 nm. The condition was optimized by orthogonal design. The results showed that chloroform： acetone=10 ： 3 as developer, the calibration curve was linear（ r=0. 9986 ）in the range of 20-200 tx L/mL, and the regression equation was Y= 0. 007 0C ＋ 0. 031 7. The content of triterpenoids in the bark of Nerium oleander L was about 1.01%. The spectrophotometry is suitable for the determination of total triterpenoids from Nerium oleander L. This method is convenient, stable and reproducible. |
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| Keywords: | Nerium oleander L triterpenoids spectrophotometry thinlayer chromatography |
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