雏鹅新型病毒性肠炎病原快速检测技术的建立及应用

根据小鹅瘟病毒(GPV)和鹅源禽腺病毒(FAV)基因组序列特征分别设计了各自的PCR引物，经对反应条件的优化建立了能同时检测两种病毒的二重PCR方法．该法能从雏鹅新型病毒性肠炎和小鹅瘟疫苗株以及分离的鹅源腺病毒蚀斑克隆株分别扩增出相应的特异性产物，而对其它常见鹅病的病原及正常鹅胚肝组织的扩增结果均为阴性；对来自广西不同地方的36份临床病例样品的检测发现，其中具有雏鹅新型病毒性肠炎典型病变的22份样品均可检出GPV和FAV两种病毒．其它的有4份只检出GPV，3份只检出FAV；12份健康雏鹅肝组织中仅有一份检测到GPV；健康成鹅泄殖腔中GPV和FAV的检出率分别达44．29％、37．14％二者都有的占1．43％．结果表明，雏鹅新型病毒性肠炎的病原可能包括GPV和FAV两种病毒；建立的PCR诊断技术具有快速、敏感、特异的特点，可用于该病征的临床快速鉴别诊断以及流行病学的调查研究．

The development and applications of rapid detection of pathogens of gosling new type viral enteritis

Two sets of PCR primers were designed to amplify specific DNA fragment from the genomes of GPV and FAV respectively for the detections of pathogens of gosling new type viral enteritis (GNTVE). The specific PCR products were amplified respectively by the corresponding primers from these reference strains, but not from other comment pathogens of gosling; 36 field samples from different areas of Guangxi were detected by the developed PCR-based technique. 22 of the samples were positive of both GPV and FAV, while 4 samples were positive of GPV and 3 samples were positive of FAV. The results demonstrated that GNTVE maybe caused by both GPV and FAV, and the developed PCR-based technique is a rapid, sensitive, specific and reliable tool for the diagnosis of GNTVE and the epidemiological survey in the field.