中华按蚊的分子鉴定标准

中华按蚊（Anopheles sinensis）是重要的传疟媒介之一,广泛分布于中国和东南亚,它的形态特征和赫坎按蚊种团（Anopheles hyrcanus Group）其他蚊种十分近似,建立中华按蚊的分子鉴定标准十分重要。本研究从实验室中华按蚊品系的一个个体测序了线粒体DNA COI和COII基因,以及核糖体DNA D2、D3和ITS2位点,测序片段长度分别为658、663、551、365、556 bp。这些序列和近缘蚊种对应序列分别用最大似然法构建系统发育树,结果表明中华按蚊的序列均聚合为同一支。将测序的中华按蚊COII序列和已知蚊种的对应序列比对发现其中的保守位点分别是第133位点C,321位点C,382位点C,435位点T,516位点A和651位点C,线粒体DNA COI和COII基因序列的碱基组成具有AT偏向性,分别占69.15%和74.96%。提交了5条序列到NCBI数据库,其中核糖体DNA D2位点序列是首次在中华按蚊中公开报道。

The Molecular Identification Markers of Anopheles sinensis

Anopheles sinensis is one of most important malaria vector species and widely distributed in China and southeastern Asia. It is quite difficult to distinguish it from other species in Anopheles hyrcanus Group in morphology therefore, it is necessary to establish the molecular identification standard through setting up a set of molecular markers. In the present study, we sequenced the mtDNA COI and COII genes, and rDNA D2, D3 and ITS2 loci using an individual of Anopheles sinensis laboratory strain. The sequence lengths obtained for the five loci are 658, 663, 551, 365 and 556 bp, respectively. The comparison of the sequenced COII with corresponding sequences of other mosquito species identified six conserved nucleotide loci, and they are C at nucleotide, C at 321, C at 382, T at 435, A at 516 and C at 651. The COI and COII sequences showed AT usage bias with AT accounting up 69.150% and 74.96 %, respectively. The sequenced five sequences were submitted to NCBI with access numbers, and the rDNA D2 sequence was the first report for the species.