Expression of human hepatitis C virus core antigen in tobacco plants by tobacco mosaic virus-based vector system |
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Authors: | Aidong Han Yule Liu Li Xiao Liangyi Kang Yuman Zhang Dongtian Li Bo Tian |
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Institution: | (1) Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China;(2) Tianjin Medical University, 300070 Tianjin, China |
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Abstract: | Tobacco mosaic virus (TMV) has the potential to highly express foreign gene. A novel TMV-basedin trans expression system was constructed. A TMV mutant TSHc had its coat protein replaced with hepatitis C virus (HCV) core antigen
gene. Anotherr TMV mutant TSBD was replicase-defective. Coinfection of the two mutants could cause systemic infection in tobacco
plants byin trans complementation of their functions. TSHc could effectively replicate and assemble to viral particles, which were a little
longer than that of wild-type TMV. HCV core antigen was expressed in whole tobacco plants. A similar expression level of HCV
core antigen was detected on serial passages, which suggested that this viral expression system be stable. |
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Keywords: | TMV vector complementation systemic infection HCV core antigen |
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