| 新疆4个绵羊群体MHC-DRB1基因外显子2多态性的遗传分析 |
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| 引用本文： | 陶金陵,陈明辉,孙延星,蒋文生,李仁燕,申红,贾斌.新疆4个绵羊群体MHC-DRB1基因外显子2多态性的遗传分析[J].石河子大学学报,2010,28(3):315-318. |
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| 作者姓名： | 陶金陵 陈明辉 孙延星 蒋文生 李仁燕 申红 贾斌 |
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| 作者单位： | 陶金陵,陈明辉(新疆兵团农九师畜牧兽医工作站,额敏县,834600);孙延星(新疆兵团农五师畜牧兽医工作站,博乐,833400);蒋文生(新疆兵团农三师畜牧兽医工作站,图木舒克,844618);李仁燕,申红,贾斌(石河子大学动物科技学院,石河子,832003) |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 采用PCR-RFLP的方法研究了哈萨克羊、多浪羊、中国美利奴羊(新疆军垦型)及哈萨克羊与中国美利奴羊(新疆军垦型)杂交一代的MHC-DRB1基因外显子2遗传多态性。结果显示,4个绵羊群体的MHC-DRB1基因外显子2表现出丰富的多态性,共检测到28种基因型和14个等位基因,其在4个绵羊群体间的分布存在不一致。对4个群体的遗传参数检测结果显示,MHC-DRB1基因外显子2的基因杂合度较大,遗传变异程度较高。根据MHC-DRB1基因外显子2在5个酶切位点的等位基因频率对4个绵羊群体进行了聚类分析,结果反映了4个群体间就MHC多态性建立的遗传关系,并初步探讨了4个群体间抗病性的相互关系。
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| 关 键 词： | 绵羊 |
Genetic Polymorphism Analysis of MHC-DRB1 Exon 2 in Four Sheep Populations |
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| TAO JINling,CHEN Minghui,SUN Yanxing,JIANG Wensheng,LI Renyan,SHEN Hong,JIA Bin.Genetic Polymorphism Analysis of MHC-DRB1 Exon 2 in Four Sheep Populations[J].Journal of Shihezi University(Natural Science),2010,28(3):315-318. |
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| Authors: | TAO JINling CHEN Minghui SUN Yanxing JIANG Wensheng LI Renyan SHEN Hong JIA Bin |
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| Institution: | TAO JINling1,CHEN Minghui1,SUN Yanxing2,JIANG Wensheng3,LI Renyan4,SHEN Hong4,JIA Bin4 (1 Animal Husb,ry , Veterinary Station at Agricultural Construction Division 9 in Xinjiang,Emin County 834600,China,2 Animal Husb,ry , Veterinary Station at Agricultural Construction Division 5 in Xinjiang,Bole 833400,3 Animal Husb,ry , Veterinary Station at Agricultural Construction Division 3 in Xinjiang,Tumushuke 844618,4 College of Animal Science , Technology,Shihezi University,Shihezi 832003... |
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| Abstract: | The polymorphism of MHC-DRB1 exon 2 was detected by the method of PCR-RFLP in four sheep populations: Chinese Merino sheep,Kazakh sheep,Duolang sheep and the hybridization generation of Kazakh sheep and Chinese Merino sheep.The result showed that there were rich polymorphism of MHC-DRB1 exon 2 in four sheep populations.A total of 14 alleles and 28 genotypes was obtained,which differed in the four populations.And a high degree of polymorphism of MHC-DRB1 exon 2 was observed in the populations in terms of the... |
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| Keywords: | MHC-DRB1 PCR-RFLP |
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