Isolation, from anti-dinitrophenyl sera taken at the beginning of immunization, of a protein combining with the hapten and which is not related to immunoglobulins]

A protein, not antigenically related to the immunoglobulins, has been isolated by affinity chromatography from the serum of rabbits immunized with dinitrophenylated human gamma-globulin. Its concentration in the serum reaches maximum during the first three days which follow the injection. On "Sephadex G-200", it is eluted after the ribonuclease. Its electrophoretic mobility is slower than that of the IgG.