| 3株果实采后葡萄孢属真菌ITS区rDNA序列与碳源代谢指纹图谱分析 |
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| 引用本文： | 姚婷,陈其葳,张燕,张萌,何欣萌,王友升.3株果实采后葡萄孢属真菌ITS区rDNA序列与碳源代谢指纹图谱分析[J].北京工商大学学报(自然科学版),2017,35(4):49-55. |
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| 作者姓名： | 姚婷 陈其葳 张燕 张萌 何欣萌 王友升 |
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| 作者单位： | 北京工商大学 北京食品营养与人类健康高精尖创新中心/食品质量与安全北京实验室, 北京 100048,北京工商大学 北京食品营养与人类健康高精尖创新中心/食品质量与安全北京实验室, 北京 100048,北京工商大学 北京食品营养与人类健康高精尖创新中心/食品质量与安全北京实验室, 北京 100048,北京工商大学 北京食品营养与人类健康高精尖创新中心/食品质量与安全北京实验室, 北京 100048,北京工商大学 北京食品营养与人类健康高精尖创新中心/食品质量与安全北京实验室, 北京 100048,北京工商大学 北京食品营养与人类健康高精尖创新中心/食品质量与安全北京实验室, 北京 100048 |
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| 基金项目： | “十二五”国家科技支撑计划项目(2015BAD16B06)；国家自然科学基金资助项目(31471626)。 |
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| 摘 要： | 从采后贮藏过程中发病的草莓、蓝莓和樱桃果实中分离到3株丝状真菌138#、233#和366#。综合形态学特征观察与ITS区rDNA序列分析结果,可确定3株真菌均为葡萄孢属病原菌(Botrytis)。分子鉴定进化树和碳源代谢聚类分析结果,都得到菌株138#与366#在一个大的分支上,而菌株233#在一个分支上。菌株138#鉴定结果与Botrytis cinerea (KJ937044.1)在同一分支上亲缘性达100%,且与366#亲缘性达99%；菌株233#鉴定结果与Botrytis elliptica(EU519207.1) 在同一分支上且亲缘性达100%。95种碳源代谢指纹图谱分析的结果表明,3株葡萄孢属病原菌对吐温80、N-乙酰-D-半乳糖胺、N-乙酰-D-葡萄糖胺和苦杏仁苷等67种碳源的代谢能力相同,包括56种最适碳源、1种可利用碳源和10种不可利用碳源,Biolog系统中鉴定出3株丝状真菌均与B. cinerea Pers.BGA相近,其中菌株233#与B. cinerea的代谢指纹图谱最为相近。
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| 关 键 词： | 果实 葡萄孢菌 ITS区rDNA序列分析 碳源代谢指纹图谱 |
| 收稿时间： | 2017/4/21 0:00:00 |
Analysis of rDNA ITS and Carbon Metabolic Fingerprinting of Botrytis Nees Isolated from Three Fruits |
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| YAO Ting,CHEN Qiwei,ZHANG Yan,ZHANG Meng,HE Xinmeng and WANG Yousheng.Analysis of rDNA ITS and Carbon Metabolic Fingerprinting of Botrytis Nees Isolated from Three Fruits[J].Journal of Beijing Technology and Business University:Natural Science Edition,2017,35(4):49-55. |
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| Authors: | YAO Ting CHEN Qiwei ZHANG Yan ZHANG Meng HE Xinmeng and WANG Yousheng |
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| Institution: | Beijing Advanced Innovation Center for Food Nutrition and Human Health/Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China,Beijing Advanced Innovation Center for Food Nutrition and Human Health/Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China,Beijing Advanced Innovation Center for Food Nutrition and Human Health/Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China,Beijing Advanced Innovation Center for Food Nutrition and Human Health/Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China,Beijing Advanced Innovation Center for Food Nutrition and Human Health/Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China and Beijing Advanced Innovation Center for Food Nutrition and Human Health/Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China |
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