| 微乳液电动色谱的运行重现性研究 |
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| 引用本文： | 谢建平,陈晓峰,张继友,陈兴国,胡之德.微乳液电动色谱的运行重现性研究[J].兰州大学学报(自然科学版),2004,40(5):48-51. |
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| 作者姓名： | 谢建平 陈晓峰 张继友 陈兴国 胡之德 |
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| 作者单位： | 1. 兰州大学,化学化工学院,甘肃,兰州,730000;周口师范学院,化学系,河南,周口,466000
2. 兰州大学,化学化工学院,甘肃,兰州,730000 |
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| 基金项目： | 国家自然科学基金(20275014)，河南省教育厅自然科学研究(200410478004) |
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| 摘 要： | 通过对12个氨基酸的4-氯-7-硝基苯-氧二氮杂茂(NBD-C1)衍生物在使用同一个微乳液缓冲液和相同的仪器操作参数下连续27次运行的分析，考察了其迁移窗口：迁移顺序以及部分衍生物的相对标准偏差(n=6)、分辨率以及理论塔板数的变化，并对影响运行重现性的原因进行了分析，归纳为3个主要方面：温度的影响，微乳液本身形成和稳定过程的影响和毛细管壁变化的影响．
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| 关 键 词： | 运行重现性 微乳液电动色谱 激光诱导荧光 氨基酸 4-氯-7-硝基苯-氧二氮杂茂 |
| 文章编号： | 0455-2059(2004)05-0048-04 |
A study on run-to-run reproducibility in microemulsion electrokinetic chromatography |
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| XIE Jian-ping.A study on run-to-run reproducibility in microemulsion electrokinetic chromatography[J].Journal of Lanzhou University(Natural Science),2004,40(5):48-51. |
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| Authors: | XIE Jian-ping |
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| Institution: | XIE Jian-ping~ |
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| Abstract: | Run-to-run reproducibility in microemulsion electrokinetic chromatography was investigated and tested with separation of twelve amino acids by microemulsion electrokinetic chromatography combined with laser-induced fluorescence detection after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol(NBDCl). In a batch of 27 runs using the same microemulsion buffer and same operation parameters, theoretical plates, resolutions, RSD(n=6), migration window and order were discovered to be changing and their causes were also analyzed. |
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| Keywords: | run-to-run reproducibility microemulsion electrokinetic chromatography laser-induced fluorescence amino acids 4-choro-7-nitrobenzo-2-oxa-1 3-diazol |
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