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| 基于水溶性共轭高分子构象效应的凝血酶检测 | |
| 引用本文： | 刘兴奋,王丽华,宋世平,樊春海,黄维.基于水溶性共轭高分子构象效应的凝血酶检测[J].南京邮电大学学报(自然科学版),2008,28(1):59-63. |
| 作者姓名： | 刘兴奋 王丽华 宋世平 樊春海 黄维 |
| 作者单位： | 1. 南京邮电大学,江苏省有机电子与信息显示重点实验室,江苏,南京,210046;南京邮电大学,信息材料与纳米技术研究院,江苏,南京,210003 2. 中国科学院,上海应用物理研究所,上海,201800 3. 南京邮电大学,江苏省有机电子与信息显示重点实验室,江苏,南京,210046;南京邮电大学,信息材料与纳米技术研究院,江苏,南京,210003;中国科学院,上海应用物理研究所,上海,201800 |
| 基金项目： | 国家自然科学基金 , 南京邮电大学引进人才科研启动基金 |
| 摘 要： | 介绍了水溶性共轭高分子和核酸适体(aptamer)在生物传感检测中的应用.提出了一种基于aptamer和水溶性聚噻吩的构象效应进行凝血酶检测的比色方法.结果表明,该方法具有较高的选择性和灵敏度,肉眼目测最低可检测63 nM凝血酶. |
| 关 键 词： | 共轭高分子 生物传感器 核酸适体 凝血酶 蛋白质 |
| 文章编号： | 1673-5439(2008)01-0059-05 |
| 修稿时间： | 2007年11月6日 |
Detection of Thrombin Based on the Conformational Effects of Water-soluble Conjugated Polymer |
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| LIU Xing-fen,WANG Li-hua,SONG Shi-ping,FAN Chun-hai,HUANG Wei.Detection of Thrombin Based on the Conformational Effects of Water-soluble Conjugated Polymer[J].Journal of Nanjing University of Posts and Telecommunications,2008,28(1):59-63. | |
| Authors: | LIU Xing-fen WANG Li-hua SONG Shi-ping FAN Chun-hai HUANG Wei |
| Institution: | LIU Xing-fen1,2,WANG Li-hua3,SONG Shi-ping3,FAN Chun-hai1,3,HUANG Wei1,21.Jiangsu Key Lab for Organic Electronics & Information Displays,Nanjing University of Posts , Telecommunications,Nanjing 210046,China2.Institute of Advanced Materials,Nanjing 210003,China3.Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China |
| Abstract: | We introduce the application of water-soluble conjugated polymers and aptamer in the detection of biomolecules. Then a colorimetric protocol based on aptamer and conformational effects of water-soluble polythiophene is reported. It is demonstrated that the protein sensor we designed is highly selective and sensitive, and the lowest detection limit with naked eyes is 63 nM. |
| Keywords: | Conjugated polymer Biosensors Aptamer Thrombin Protein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |