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Localization of genes for lateral branch and female sex expression and construction of a molecular linkage map in cucumber (Cucumis sativus L. ) with RAPD markers
作者姓名:LI Xiaozun  PAN Junsong  WANG Gang  TIAN Libo  SI Longting  WU Aizhong  CAI Run
作者单位:Agriculture and Biology School,Shanghai Jiaotong University,Shanghai 201101,China,Agriculture and Biology School,Shanghai Jiaotong University,Shanghai 201101,China,Agriculture and Biology School,Shanghai Jiaotong University,Shanghai 201101,China,Horticulture School,Shenyang Agricultural University,Shenyang 110161,China,Horticulture School,Shenyang Agricultural University,Shenyang 110161,China,Shanghai Academy of Agricultural Science,Shanghai 201106,China,Agriculture and Biology School,Shanghai Jiaotong University,Shanghai 201101,China
摘    要:A cucumber ( Cucumis sativus L. ) molecular linkage map, including 79 random-amplified polymorphic DNAs (RAPD)and two genes , lb for lateral branch and f for female sex expression, is constructed from a cross between a line, S52, with weak lateral growing ability and staminate from Dabieshan Mountains area in China and another line, S06, with strong lateral growing ability and gynoecious from Europe. The map contains nine linkage groups and spans 1110.0 cM with an average distance of 13.7 cM between loci. The lb locus is located in a longer linkage group LG-2 and flanked by two markers, OP-Q5-1 and OP-M-2-2, at 9.3 cM and 15.9 cM, respectively. In the meantime, the RAPD loci, OP-Q5-2 and BC151, in a short linkage group were found to flank f at 13.7 cM and 13.4 cM,respectively. The construction of RAPD map has paved a way for further study of the genes for lateral branch, female sex expression and other agronomic traits in cucumber.

关 键 词:cucumber  (Cucumis  sativus  L.  )    lateral  branch    gynoecy    RAPD    genetic  mapping.

Localization of genes for lateral branch and female sex expression and construction of a molecular linkage map in cucumber (Cucumis sativus L. ) with RAPD markers
LI Xiaozun,PAN Junsong,WANG Gang,TIAN Libo,SI Longting,WU Aizhong,CAI Run.Localization of genes for lateral branch and female sex expression and construction of a molecular linkage map in cucumber (Cucumis sativus L. ) with RAPD markers[J].Progress in Natural Science,2005,15(2):143-148.
Authors:LI Xiaozun  PAN Junsong  WANG Gang  TIAN Libo  SI Longting  WU Aizhong  CAI Run
Institution:1. Agriculture and Biology School,Shanghai Jiaotong University,Shanghai 201101,China
2. Horticulture School,Shenyang Agricultural University,Shenyang 110161,China
3. Shanghai Academy of Agricultural Science,Shanghai 201106,China
Abstract:A cucumber ( Cucumis sativus L. ) molecular linkage map, including 79 random-amplified polymorphic DNAs (RAPD)and two genes , lb for lateral branch and f for female sex expression, is constructed from a cross between a line, S52, with weak lateral growing ability and staminate from Dabieshan Mountains area in China and another line, S06, with strong lateral growing ability and gynoecious from Europe. The map contains nine linkage groups and spans 1110.0 cM with an average distance of 13.7 cM between loci. The lb locus is located in a longer linkage group LG-2 and flanked by two markers, OP-Q5-1 and OP-M-2-2, at 9.3 cM and 15.9 cM, respectively. In the meantime, the RAPD loci, OP-Q5-2 and BC151, in a short linkage group were found to flank f at 13.7 cM and 13.4 cM,respectively. The construction of RAPD map has paved a way for further study of the genes for lateral branch, female sex expression and other agronomic traits in cucumber.
Keywords:cucumber (Cucumis sativus L  )  lateral branch  gynoecy  RAPD  genetic mapping
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