Cortisol resistant RPMI-1788 lymphocytes become sensitive to cortisol subsequent to a 24-h incubation period in medium containing purified human transcortin |
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Authors: | L Amaral S Werthamer |
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Institution: | (1) Department of Pathology, The Bronx-Lebanon Hospital Center, 1276 Fulton Avenue, 10456 Bronx, New York, USA;(2) Department of Pathology, Methodist Hospital of Brooklyn, 1121 Brooklyn, New York, USA |
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Abstract: | Summary RPMI-1788 lymphocytes (a human cell line) are resistant to cortisol in vitro. Prior incubation for a minimum of 24 h a medium which contains purified human transcortin at a concentration of 50 g/ml renders these cells sensitive to the inhibitory action of cortisol as regards the synthesis of DNA. Only the transcortin-exposed cells contain a cortisol binding species whose sedimentation behavior in a sucrose gradient is identical to that of transcortin.This work was supported in part by grant ET-47 from the American Cancer Society. I thank Mr D. Pina and Dr R. Khaund for their excellent technical assistance. |
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