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| 葡萄幼胚培养技术的研究(Ⅱ)——接种时间及处理方法对胚培养的影响 | |
| 引用本文： | 刘海琳,尚忠海,崔玲华,廖康,陈力耕. 葡萄幼胚培养技术的研究(Ⅱ)——接种时间及处理方法对胚培养的影响[J]. 河南科学, 2006, 24(6): 844-848 |
| 作者姓名： | 刘海琳 尚忠海 崔玲华 廖康 陈力耕 |
| 作者单位： | 1. 河南科技大学,林业职业学院,河南,洛阳,471002;浙江大学,农生学院果树研究所,杭州,310029 2. 河南科技大学,林业职业学院,河南,洛阳,471002;河南农业大学,林学园艺学院,郑州,450002 3. 河南科技大学,林业职业学院,河南,洛阳,471002 4. 新疆农业大学,园艺学院,乌鲁木齐,830052 5. 浙江大学,农生学院果树研究所,杭州,310029 |
| 摘 要： | 选用花后35￣60d的巨峰、里扎马特和无核白葡萄,取其幼胚接种于不同激素水平M S培养基上,进行胚培养,以研究接种时间及处理方法对胚培养的影响.结果表明对花后60d的里扎玛特和巨峰的幼胚进行胚培养,其发芽率和成苗率显著高于层积处理后催芽播种的种子的发芽率与出苗率.低温和横切处理能有效地促进葡萄幼胚的萌发.花后60d的巨峰和里扎马特的发育率和成苗率最高;花后60d的无核白的幼胚发育率最高,花后50d的幼胚成苗率最高. |
| 关 键 词： | 胚培养 幼胚 接种时间 |
| 文章编号： | 1004-3918(2006)05-0844-05 |
| 收稿时间： | 2006-07-10 |
| 修稿时间： | 2006-07-10 |
The Study on the Technique of Embryo Culture of Grape (Ⅱ) |
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| LIU Hai-lin,SHANG Zhong-hai,CUI Ling-hua,LIAO Kang,CHENG Li-geng. The Study on the Technique of Embryo Culture of Grape (Ⅱ)[J]. Henan Science, 2006, 24(6): 844-848 | |
| Authors: | LIU Hai-lin SHANG Zhong-hai CUI Ling-hua LIAO Kang CHENG Li-geng |
| Affiliation: | 1. College of Forestry Vocation, Henan Sci-Technology University, Luoyang 471002, China; 2. Fruit Tree Research Institute of Horticulture Department of Agriculture and Tech-Biology College,Zhejiang University, Hangzhou 310029, China; 3. College of Forestry and Horticulture, Henan Agricultural University, Zhengzhou 450002, China; 4. College of Horticulture, Xinjiang Agricultural University, Urumqi 830052, China |
| Abstract: | 35~60 days after florescence,embryo of Kyoho(V.vinifera × V.labrusce cv.),Rizamat(V.vinifera cv.) and Thompson Seedless(V.vinifera cv.Sultanina) grapes were cultured on MS medium supplemented with different levels of concentrations of GA3,IAA and 6-BA in order to study the techniques of embryo rescue.The results indicated that the germination rate and seedling rate of the embryo culture were higher than those of insemination.The low temperature and the cutting treatments were affected to the young embryos germination.The highest development rate and germinating rate of Rizamat's and Kyoho's embryos were gotten by the time which was 60 days after florescence.The best period of inoculation of Thompson Seedless' embryos was 50 days after florescence. |
| Keywords: | embryo culture young embryo inoculation time |
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