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测定血小板衍生生长因子活性方法的改进
引用本文:王湛,李充璧,周明海,王利春,阿丽娅. 测定血小板衍生生长因子活性方法的改进[J]. 内蒙古大学学报(自然科学版), 2004, 35(1): 37-39
作者姓名:王湛  李充璧  周明海  王利春  阿丽娅
作者单位:内蒙古大学生物工程中心,呼和浩特 010021
摘    要:以BALB/C小鼠胚胎成纤维细胞(BALB/C3T3)作为靶细胞,经20mmol/L醋酸钠平衡后的CM—Sepharose(CMS)吸附过的胎牛血清作为基础培养血清,应用[3-(4,5-dimethylthi—azolyl)-2,5-diphenyhetrazolium bromide,MTT]方法测定血小板源生长因子(platelet—derived growth factor,PDGF)诱导细胞增殖的生物活性,结果表明,该方法具有良好的量效关系,且精确度、重现性均较高。

关 键 词:CM—Sepharose BALB/C 3T3细胞 血小板源生长因子(PDGF)
文章编号:1000-1638(2004)01-0037-03
修稿时间:2002-07-02

An Improved Method of Determining PDGF Activity
WANG Zhan,LI Chong-bi,ZHOU Ming-hai,WANG Li-chun,A Lya. An Improved Method of Determining PDGF Activity[J]. Acta Scientiarum Naturalium Universitatis Neimongol, 2004, 35(1): 37-39
Authors:WANG Zhan  LI Chong-bi  ZHOU Ming-hai  WANG Li-chun  A Lya
Abstract:The activity of platelet-derived growth factor( PDGF)was determined by the method of 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide(MTT).BALB/C embryo fibroblast 3T3 cells were taken as the target cells.The cow foetus serum (CFS) absorbed by carboxymethylcellulose-sephose (CMS) was taken as a basic cultural serum.The results indicated that this method was more accurate and repeatable,and had a good relationship between the effects and quantities
Keywords:CMS  BLAB/C 3T3  PDGF
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