| 测定血小板衍生生长因子活性方法的改进 |
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| 引用本文： | 王湛,李充璧,周明海,王利春,阿丽娅.测定血小板衍生生长因子活性方法的改进[J].内蒙古大学学报(自然科学版),2004,35(1):37-39. |
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| 作者姓名： | 王湛 李充璧 周明海 王利春 阿丽娅 |
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| 作者单位： | 内蒙古大学生物工程中心，呼和浩特 010021 |
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| 摘 要： | 以BALB／C小鼠胚胎成纤维细胞(BALB／C3T3)作为靶细胞，经20mmol／L醋酸钠平衡后的CM—Sepharose(CMS)吸附过的胎牛血清作为基础培养血清，应用3-(4，5-dimethylthi—azolyl)-2，5-diphenyhetrazolium bromide，MTT]方法测定血小板源生长因子(platelet—derived growth factor，PDGF)诱导细胞增殖的生物活性，结果表明，该方法具有良好的量效关系，且精确度、重现性均较高。
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| 关 键 词： | CM—Sepharose BALB／C 3T3细胞 血小板源生长因子（PDGF） |
| 文章编号： | 1000-1638(2004)01-0037-03 |
| 修稿时间： | 2002年7月2日 |
An Improved Method of Determining PDGF Activity |
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| WANG Zhan,LI Chong-bi,ZHOU Ming-hai,WANG Li-chun,A Lya.An Improved Method of Determining PDGF Activity[J].Acta Scientiarum Naturalium Universitatis Neimongol,2004,35(1):37-39. |
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| Authors: | WANG Zhan LI Chong-bi ZHOU Ming-hai WANG Li-chun A Lya |
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| Abstract: | The activity of platelet-derived growth factor( PDGF)was determined by the method of 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide(MTT).BALB/C embryo fibroblast 3T3 cells were taken as the target cells.The cow foetus serum (CFS) absorbed by carboxymethylcellulose-sephose (CMS) was taken as a basic cultural serum.The results indicated that this method was more accurate and repeatable,and had a good relationship between the effects and quantities |
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| Keywords: | CMS BLAB/C 3T3 PDGF |
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