| 碱性纤维素酶高产菌株Bacillus sp.CY1-3的诱变选育 |
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| 引用本文： | 蔡勇,阿依木古丽. 碱性纤维素酶高产菌株Bacillus sp.CY1-3的诱变选育[J]. 西北民族学院学报, 2007, 28(2): 13-16 |
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| 作者姓名： | 蔡勇 阿依木古丽 |
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| 作者单位： | 西北民族大学理科实验中心 西北民族大学生命科学与工程学院 甘肃兰州 甘肃兰州 |
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| 基金项目： | 西北民族大学校科研和教改项目;西北民族大学校科研和教改项目 |
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| 摘 要： | 以一株产单组分羧甲基纤维素酶芽孢杆菌Bacillus sp.CY1-3为出发菌,经紫外线和亚硝基胍复合诱变处理后,其纤维素酶产量达到了53.86 U/mL,是出发菌株的4倍.研究发现最优的紫外辐射时间和亚硝基胍浓度分别是4min和0.06%.
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| 关 键 词： | 纤维素酶 酶活力 诱变 芽孢杆菌CY1-3 |
| 文章编号： | 1009-2102(2007)02-0013-04 |
| 修稿时间： | 2007-03-25 |
Mutagenesis of Alkaline Cellulase High-producing Bacillus Strain sp.CY1-3 |
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| CAI Yong,Ayimuguli. Mutagenesis of Alkaline Cellulase High-producing Bacillus Strain sp.CY1-3[J]. Journal of Northwest Minorities University(Natural Science ), 2007, 28(2): 13-16 |
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| Authors: | CAI Yong Ayimuguli |
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| Abstract: | A strain of Bacillus sp.CY1-3 which could produce endo-1,4-β-glucanase was mutagenized by UV and NTG,gave a cellulase yield of 53.86 U/mL which was 4 times as that of original strain.Results showed that the optimum UV irradiation time was four minutes and the best NTG concentration was 0.06%. |
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| Keywords: | cellulase activity of enzyme mutagenesis Bacillus sp.CY1-3 |
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