Rapid assessment of acid phosphatase activity in the mycorrhizosphere and in arbuscular mycorrhizal fungal hyphae |
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Authors: | Yongchun Song Xiaolin Li Gu Feng Fusuo Zhang Christie Peter |
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Institution: | (1) Department of Plant Nutrition, China Agricultural University, 100094 Beijing, China;(2) Agricultural and Environmental Science Department, The Queen’s University of Belfast, Newforge Lane, BT9 SPX Belfast, UK |
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Abstract: | A pot experiment has been carried out under controlled conditions to study the possibility of applying the technique ofin vivo staining for acid phosphatase activity on the roots of mycorrhizal plants and arbuscular mycorrhizal hyphae. The pots had
5 compartments. The central root compartment was separated from the two adjacent hyphal compartments using nylon nets of 30
μm mesh, and the two hyphal compartments were separated from the two outermost compartments with 0.45 μm membranes. Red clover
was grown in the root compartment and was either inoculated with the arbuscular mycorrhizal fungus (AMF)Glomus mosseae or unlnoculated. Sodium phytate was applied to all compartments. The results show that AMF can increase acid phosphatase
activity of clover roots. The plant roots acquired deep red “mycorrhizal prints”. The external hyphae also had obvious “hyphal
prints” on the test papers, indicating the ability of mycorrhizal hyphae to release acid phosphatase. |
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Keywords: | acid phosphatase arbuscular mycorrhizal hyphae red clover |
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