光肩星天牛对糖槭挥发物的EAG及嗅觉行为反应

[目的]分析影响光肩星天牛定位寄主的糖槭挥发物成分,解析挥发物在光肩星天牛搜寻和定位寄主过程中的作用,为光肩星天牛的综合防控提供依据.[方法]利用热脱附气质联用法(thermal desorption gas chromatog-raphy-mass spectrometry,TCT-GC-MS)分析鉴定糖槭挥发物组分...

EAG and olfactory behavioral responses of Anoplophora glabripennis(Coleoptera:Cerambycidae)to volatiles of Acer saccharum

【Objective】This study was conducted to identify the volatile components of Acer saccharum,explore how the volatile constituents of Acer saccharum affect the host position of Anoplophora glabripennis,analyze the role of host volatiles in the search for and host positioning of Anoplophora glabripennis,and provide new ideas and a theoretical basis for the comprehensive prevention and control of Anoplophora glabripennis infestations.【Method】The volatile components of Acer saccharum were identified by TCT-GC-MS.The electrophysiological and behavioral responses of Anoplophora glabripennis to Acer saccharum volatiles were determined by the electroantennogram(EAG)and Y-tube olfactometer.The main volatiles affecting the search and host location of A.glabripennis were identified by multiple comparison methods,an independent sample t test,and a chi-square test analysis.【Result】The volatiles released from Acer saccharum included 34 volatile compounds,the main components of which were esters(61.67%),alkenes(12.99%),acids(5.58%)and alcohols(5.02%),respectively.There were electrophysiological and behavioral responses to 12 volatile compounds.The electrophysiological and behavioral responses of the female and male A.glabripennis adults were significantly different to the same concentration of different volatiles,the same volatiles,and different concentrations of the same volatiles.Male and female A.glabripennis adults exhibited obvious electrophysiological responses to hexyl acetate,diisobutyl adipate,diisobutyl phthalate,hexenyl acetate,geranyl propionate,cis-3-hexenol,2-hexyl-1-decanol,ocimene and squalene.Among these,the female and male adults of A.glabripennis showed a significant attraction to hexyl acetate,hexenyl acetater,geranyl propionate,cis-3-hexenol,and 2-hexyl-1-nonanol,while ocimene and squalene had significant repellent effects.Female adults of A.glabripennis were significantly attracted to diisobutyl adipate and lauric acid,but these compounds elicited no behavioral response from male adults.【Conclusion】Female and male adults of A.glabripennis are strongly attracted to hexenyl acetater,geranyl propionate,cis-3-hexenol,and 2-hexyl-1-nonanol,while ocimene and squalene show good repellent qualities.Diisobutyl adipate and lauric acid were significantly attractive to female adults of A.glabripennis,but male adults showed no behavioral response.