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光肩星天牛对糖槭挥发物的EAG及嗅觉行为反应
引用本文:马晓乾,葛君,王琪,赵红盈,孙妍,高宇,申国涛,于文喜.光肩星天牛对糖槭挥发物的EAG及嗅觉行为反应[J].南京林业大学学报(自然科学版),2021,45(1):123-130.
作者姓名:马晓乾  葛君  王琪  赵红盈  孙妍  高宇  申国涛  于文喜
作者单位:1.黑龙江省林业科学院森林保护研究所,黑龙江 哈尔滨 1500812.东北林业大学林学院,黑龙江 哈尔滨 150040
基金项目:黑龙江省森工总局应用研究重点项目(SGZJY2013010);黑龙江省省级基本科研业务费项目(20170013)。
摘    要:【目的】分析影响光肩星天牛定位寄主的糖槭挥发物成分,解析挥发物在光肩星天牛搜寻和定位寄主过程中的作用,为光肩星天牛的综合防控提供依据。【方法】利用热脱附气质联用法(thermal desorption gas chromatography-mass spectrometry,TCT-GC-MS)分析鉴定糖槭挥发物组分,通过触角电位仪和“Y”型嗅觉仪测定光肩星天牛对槭树挥发物的触角电位(EAG)及嗅觉行为反应。采用多重比较、独立样本t检验和卡方检验分析等方法,明确影响光肩星天牛搜寻和定位寄主的主要挥发物。【结果】经鉴定,糖槭具有34种挥发性化合物,其主要成分为酯类、烯类、酸类和醇类,分别占61.67%、12.99%、5.58%和5.02%。光肩星天牛对所测定的12种糖槭挥发物均有一定的EAG及嗅觉行为反应,在不同挥发物间、同种挥发物不同浓度间、同种挥发物同一浓度雌、雄光肩星天牛成虫间EAG及嗅觉行为反应存在一定的差异。光肩星天牛雌、雄成虫对乙酸己酯、己二酸二异丁酯、邻苯二甲酸二异丁酯、乙酸叶醇酯、丙酸叶醇酯、顺-3-己烯醇、2-己基-1-癸醇、罗勒烯和角鲨烯具有明显的EAG反应;乙酸己酯、乙酸叶醇酯、丙酸叶醇酯、顺-3-己烯醇和2-己基-1-癸醇对光肩星天牛雌、雄成虫均具有较强的引诱作用,罗勒烯和角鲨烯具有较强的驱避作用;己二酸二异丁酯和月桂酸对光肩星天牛雌成虫具有引诱作用,对雄成虫无明显的趋性引诱作用。【结论】糖槭挥发物中乙酸己酯、乙酸叶醇酯、丙酸叶醇酯、顺-3-己烯醇和2-己基-1-癸醇对光肩星天牛雌、雄成虫均具有较强的引诱作用,罗勒烯和角鲨烯具有较强的驱避作用。己二酸二异丁酯和月桂酸对光肩星天牛雌成虫具有引诱作用,对雄成虫无明显的趋性作用。

关 键 词:光肩星天牛  糖槭  挥发物  热脱附气质联用法(TCT-GC-MS)  触角电位(EAG)  嗅觉行为  
收稿时间:2019-07-31

EAG and olfactory behavioral responses of Anoplophora glabripennis(Coleoptera:Cerambycidae)to volatiles of Acer saccharum
MA Xiaoqian,GE Jun,WANG Qi,ZHAO Hongying,SUN Yan,GAO Yu,SHEN Guotao,YU Wenxi.EAG and olfactory behavioral responses of Anoplophora glabripennis(Coleoptera:Cerambycidae)to volatiles of Acer saccharum[J].Journal of Nanjing Forestry University(Natural Sciences ),2021,45(1):123-130.
Authors:MA Xiaoqian  GE Jun  WANG Qi  ZHAO Hongying  SUN Yan  GAO Yu  SHEN Guotao  YU Wenxi
Institution:(Institute of Forest Protection,Heilongjiang Academy of Forestry,Harbin 150081,China;College of Forestry,Northeast Forestry University,Harbin 150040,China)
Abstract:【Objective】This study was conducted to identify the volatile components of Acer saccharum,explore how the volatile constituents of Acer saccharum affect the host position of Anoplophora glabripennis,analyze the role of host volatiles in the search for and host positioning of Anoplophora glabripennis,and provide new ideas and a theoretical basis for the comprehensive prevention and control of Anoplophora glabripennis infestations.【Method】The volatile components of Acer saccharum were identified by TCT-GC-MS.The electrophysiological and behavioral responses of Anoplophora glabripennis to Acer saccharum volatiles were determined by the electroantennogram(EAG)and Y-tube olfactometer.The main volatiles affecting the search and host location of A.glabripennis were identified by multiple comparison methods,an independent sample t test,and a chi-square test analysis.【Result】The volatiles released from Acer saccharum included 34 volatile compounds,the main components of which were esters(61.67%),alkenes(12.99%),acids(5.58%)and alcohols(5.02%),respectively.There were electrophysiological and behavioral responses to 12 volatile compounds.The electrophysiological and behavioral responses of the female and male A.glabripennis adults were significantly different to the same concentration of different volatiles,the same volatiles,and different concentrations of the same volatiles.Male and female A.glabripennis adults exhibited obvious electrophysiological responses to hexyl acetate,diisobutyl adipate,diisobutyl phthalate,hexenyl acetate,geranyl propionate,cis-3-hexenol,2-hexyl-1-decanol,ocimene and squalene.Among these,the female and male adults of A.glabripennis showed a significant attraction to hexyl acetate,hexenyl acetater,geranyl propionate,cis-3-hexenol,and 2-hexyl-1-nonanol,while ocimene and squalene had significant repellent effects.Female adults of A.glabripennis were significantly attracted to diisobutyl adipate and lauric acid,but these compounds elicited no behavioral response from male adults.【Conclusion】Female and male adults of A.glabripennis are strongly attracted to hexenyl acetater,geranyl propionate,cis-3-hexenol,and 2-hexyl-1-nonanol,while ocimene and squalene show good repellent qualities.Diisobutyl adipate and lauric acid were significantly attractive to female adults of A.glabripennis,but male adults showed no behavioral response.
Keywords:Anoplophora glabripennis  Acer saccharum  volatiles  TCT-GC-MS  EAG  olfactory behavior
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