木犀草素和木犀草苷对K562细胞增殖的影响

选择合适浓度的DMSO溶剂，观察和比较糖苷和苷元对K562细胞增殖的影响．采用MTT法测定DMSO、木犀草素和木犀草苷对K562细胞增殖的影响，对结果进行了统计分析．处理结果显示：DMSO作为溶剂对K562细胞增殖产生影响的最小的浓度为0．1％．梯度实验显示：木犀草素和木犀草苷同它们的抑制作用都有量效关系．结果表明：最好选用0．1％DMSO作为观察药物影响K562细胞增殖实验的助溶剂，浓度偏大或偏小都会表现出它对K562细胞增殖的影响；在0．1％DMSO中，木犀草素和木犀草苷对K562细胞增殖均可表现出抑制作用，且呈浓度依赖性；木犀草苷的作用比木犀草素强；1．15umol／L的木犀草苷在水溶液中的作用比在0．1％DMSO中的强，且差异极其显著．

Effects of Luteolin and Luteolin-7-O-glucoside on Proliferation of K562 Cells

In a tested compatible concentration of dimethylsulfoxide （ DMSO）, the effects of luteolin and luteolin-7-O-glucoside on proliferation of K562 cells had been compared. MTT assay was used for the tests, and results were statistically analysed. Results showed that DMSO in 0. 1% concentration had less effect on the proliferation of K562 cells than it in other concentrations, which made 0.1% DMSO the most compatible to solve aglycone and glycosides for further researches on their effects on the prolif- eration of K562 cells. Furthermore the dose - dependency on inhibitory effect was found in both of luteolin and luteolin-7-O-glucoside by testing in the concentrations of 1.15 × 10^-8mol/L, 1.15 × 10^-7mol/L and 1.15 × 10^-6mol/L. It could be concluded that 0.1% was the optimum concentration for DMSO being the menstruum. Both Luteolin and luteolin-7-O-glucoside had inhibitory effects on proliferation of K562 cells, but the effect of luteolin-7-O-glucoside might be much stronger.