| 猪口蹄疫病毒基因工程疫苗的工程菌的发酵研究 |
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| 引用本文： | 郭杰炎. 猪口蹄疫病毒基因工程疫苗的工程菌的发酵研究[J]. 复旦学报(自然科学版), 1998, 37(4): 496-500 |
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| 作者姓名： | 郭杰炎 |
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| 作者单位： | 复旦大学微生物和微生物工程系 |
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| 基金项目： | 八五”国家重点科技攻关项目 |
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| 摘 要： | 在猪口蹄疫病毒活性肽ＶＰ１融合蛋白基因工程菌Ｅ．ｃｏｌｉＣ５００构建成功的基础上，研究了Ｃ５００的质粒稳定性，Ｃ５００在４种不同培养基中的生长曲线，以及不同种龄，接种量，装液量，碳源氮源对菌体生长的影响。
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| 关 键 词： | 口蹄疫病毒 猪病 发酵 基因工程 疫苗 |
Fermentation Study for Expression of VP1 of Foot-and-Mouth Disease Virus in E. coli |
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| Jiang Peihong, Guo Jieyan. Fermentation Study for Expression of VP1 of Foot-and-Mouth Disease Virus in E. coli[J]. Journal of Fudan University(Natural Science), 1998, 37(4): 496-500 |
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| Authors: | Jiang Peihong Guo Jieyan |
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| Abstract: | In this study, the fermentation conditions for E. colt C500, which was transformed with an expression constructin for VP1 of Foot-and-Mouth Disease Virus (FMDV)as a fusion protein, was studied. The studies included plasmid stability, growth curve of C500 in 4 different types of medium, and the affection of different seed-ages, amounts of seed bacteria, volume of medium in Erlenmeyer flasks, carbon sources and nitrogen sources on the growth of C500. The optimal cultivation conditions were then determined, and applied to a 5-L fermentor. The biomass of C500 was raised from 4. 5 up to 30 successfully. |
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| Keywords: | E. coli C500 FMDV VP1 fusion protein fermentation |
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