一株橙色黏球菌的筛选及初步鉴定

从土样中通过菌体诱导和纤维素诱导法来富集和选择性分离黏细菌,将初筛菌株进行抗菌谱测定,挑选抑菌活性强的一株(编号为hg59)进行系统分类鉴定.结果表明:该菌株革兰氏染色阴性,营养细胞呈杆状,两头稍尖,大小(5～8)μm×(0.3～0.6)μm,黏孢子为卵圆形或椭圆形,菌落生长初期为淡黄色,渐变成橙黄色,薄而向边缘扩展,有滑动痕迹,对G+,G-菌均具有抑菌活性,其中对枯草芽孢杆菌和醋酸杆菌抑菌效果明显,对米曲霉则较差.经形态观察、生理生化测定和16SrDNA序列分析,初步鉴定为橙色黏球菌(Myxococcus fulvus strain).

Screening and Preliminary Identification of Myxococcus fulvus strain

Myxobacteria have been enriched and isolated by cell and cellulose induction methods from the soil sample.The pure strains are used for testing of antibacterial spectrum,and the strain hg59with high activity is identified.The results show that the strain is gram-negative,rod shape,size(5~8) μm×(0.3~0.6) μm,sticky spores oval or oval-shaped,light yellow colonies at initial stage of growth,gradual into orange,thin to the edge of the expansion,sliding,having antibacterial activities to the G+,G-bacteria,and the effect on Bacillus subtilis and Acetobacter is significant,but on Aspergillus oryzae is poor.With the following identifications of colony morphology,physiological and biochemical experiments and 16S rDNA sequence analysis,the strain is identified as Myxococcus fulvus.