| 西洋参悬浮细胞的超低温保存研究 |
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| 引用本文： | 李亚璞,张小兵,闫静辉.西洋参悬浮细胞的超低温保存研究[J].河北省科学院学报,2011,28(4):32-35. |
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| 作者姓名： | 李亚璞 张小兵 闫静辉 |
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| 作者单位： | 河北省科学院生物研究所,河北石家庄,050081 |
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| 摘 要： | 对西洋参悬浮细胞的超低温保存进行了初步的研究，结果表明，首先将西洋参悬浮细胞在含8％蔗糖昀西洋参液体培养基中预培养2天，然后经过10％DMSO＋8％萄葡糖的冷冻保护剂处理，以4℃（60min）、0℃（30min）、-20℃（2h）、-70℃（30min）的降温程序处理，最后投入液氯中，西洋参悬浮细胞可以生长为愈伤组织，且愈伤细织长势旺盛。
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| 关 键 词： | 西洋参 悬浮细胞 超低温保存 |
The study on cryopreservation technique for Panax quinquefolium |
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| LI Yapu,ZHANG Xiao-bing,YAN Jing-hui.The study on cryopreservation technique for Panax quinquefolium[J].Journal of The Hebei Academy of Sciences,2011,28(4):32-35. |
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| Authors: | LI Yapu ZHANG Xiao-bing YAN Jing-hui |
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| Institution: | ( Institute of Biology. Hehei Academy of Sciences. Shijiazhuung Flebei 050081 ,China ) |
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| Abstract: | The cryopreservation for suspension cells of panax quinquefolium is studied. The result shows that it is probable for the cryopreservalion for suspension cells of panax quinquefolium. First, lhey are precultured for 2 days in MS with 8% Sucrose, and cryoprotectants processing with 10% DMSO and 8% Glucose, and are freezed with 4℃ （60min） ,0℃ （30min） , -20℃（2h） ,-70℃（ （30min） , they were immersed immediately into liquid nitrogen directly at last. They can regenerate calli showing vigorous growth. |
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| Keywords: | Panax quinquefolium Suspension cells Cryopreservalio |
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