Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt |
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| Authors: | Rongzhao Fu Yufa Peng Guangcheng Cao Jiangsheng Ma Caixia Chen Liming Zhang Wenbin Li Yongru Sun |
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| Affiliation: | (1) Institute of Genetics, Chinese Academy of Sciences, 100101 Beijing, China;(2) Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100094 Beijing, China |
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| Abstract: | A cDNA-derived 200 bp fragment encoding a rabbit defensin NP-1 mature peptide was ligated into the GUS-less pBH21 to yield pBIC-35NP1. Tobacco leaf discs were genetically transformed with anAgrobacterium tumefaclens strain harbouring pBIC-3SNP1. Northern blot analysis showed that the defensin NP-1 expression cassette was normally transcribed in transgenic plants. Resistance tests demonstrated that expression of native defensin NP-1 can confer partial resistance to the bacterial wilt pathogen,Ralstonia solanacearum. |
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| Keywords: | bacterial disease-resistance rabbit defensin tobacco transgenic plant |
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