| 混合异养螺旋藻中藻蓝蛋白的分离和纯化 |
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| 引用本文： | 张义明,郭祀远,陈峰.混合异养螺旋藻中藻蓝蛋白的分离和纯化[J].华南理工大学学报(自然科学版),1999,27(4):32-36. |
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| 作者姓名： | 张义明 郭祀远 陈峰 |
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| 作者单位： | 华南理工大学轻化工研究所,广州,510640 |
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| 摘 要： | 为了从螺旋藻中分离和纯化藻蓝蛋白，研究了一种采用硫铵分步盐析，离子交换层析和凝胶过滤层析的分离纯化方法，在硫酸铵分步盐析中，通过对沉淀物的吸收光谱扫描发现，３００ｇ／Ｌ的硫酸铵溶溶能很好除去杂蛋白，而５００ｇ／Ｌ和６５０ｇ／Ｌ的硫酸铵溶液能较好离ｃ－蓝蓝蛋白和别藻蓝蛋白。
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| 关 键 词： | 分离 纯化 藻蓝蛋白 螺旋藻 |
SEPARATION AND PURIFICATION OF PHYCOCYANINS FROM SPIRULINA PLATENSIS IN MIXOTROPHIC CULTURE |
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| Zhang Yiming,Guo Siyuan,Chen Feng.SEPARATION AND PURIFICATION OF PHYCOCYANINS FROM SPIRULINA PLATENSIS IN MIXOTROPHIC CULTURE[J].Journal of South China University of Technology(Natural Science Edition),1999,27(4):32-36. |
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| Authors: | Zhang Yiming Guo Siyuan Chen Feng |
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| Abstract: | For
phycocyanin separation and purification of Spirulina platensis , a method using fraction
precipitation of ammonium sulphate, ion exchange and gel filtration chromatography was
investigated. In fraction precipitation of ammonium sulphate, it was found that by adsorption
spectra scan of precipitates, 300g/L ammonium sulphate solution could remove other proteins
well while 500g/L and 650g/L ammonium sulphate solutions could separate c_phycocyanin and
allophycocyanin well. After ion exchange chromatography and gel filtration chromatography,
finally, the absorbance ratios, reflecting the purities of c_phycocyanin and allophycocyanin,
reached 5.06 and 5.34 respectively. |
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| Keywords: | separation purification phycocyanin Spirulina platensis |
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