| 不同破壁方法对活性污泥总DNA提取效果的影响 |
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| 引用本文： | 郑雪松,李道棠,杨虹. 不同破壁方法对活性污泥总DNA提取效果的影响[J]. 上海交通大学学报, 2004, 38(5): 815-818 |
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| 作者姓名： | 郑雪松 李道棠 杨虹 |
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| 作者单位： | 上海交通大学,生命科学技术学院,上海,200240;上海交通大学,生命科学技术学院,上海,200240;上海交通大学,生命科学技术学院,上海,200240 |
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| 基金项目： | 国家自然科学基金资助项目(50278053) |
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| 摘 要： | 分别采用酶法、化学法、珠磨匀浆法和反复冻融法对活性污泥样品进行破壁，提取总DNA，并通过提取的核酸总量、纯度、片段分布情况、是否含有聚合酶链反应抑制剂以及基因间隔序列(ITS)扩增产物多态性等5个指标来评价不同的细胞破壁方法对活性污泥总DNA提取效果的影响。结果表明，化学法的优势最明显，提取的总量最多，大片段提取效果好，虽然杂质较多，但不影响聚合酶链反应，方法的偏倚性最小。
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| 关 键 词： | 活性污泥 细胞破壁 DNA提取 聚合酶链反应 |
| 文章编号： | 1006-2467(2004)05-0815-04 |
| 修稿时间： | 2003-06-30 |
Evaluation of Different DNA Extraction Methods for Activated Sludge |
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| ZHENG Xue-song,LI Dao-tang,YANG Hong. Evaluation of Different DNA Extraction Methods for Activated Sludge[J]. Journal of Shanghai Jiaotong University, 2004, 38(5): 815-818 |
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| Authors: | ZHENG Xue-song LI Dao-tang YANG Hong |
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| Abstract: | Four different DNA extraction methods for activated sludge were evaluated. They are enzymatic lysis, chemical lysis, bead mill homogenization and freeze-thawing. The effects of them were compared based on the yield, purity, molecular size distribution of the recovered DNA, and existence of PCR-inhibitor. The bias of the four methods was evaluated according to the ITS PCR products length polymorphism. The results show that the chemical lysis is most effective and has the most total DNA yield and large size fragments, the impurities in the products by chemical lysis do not inhibit the PCR, and the bias of this method is the least. |
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| Keywords: | activated sludge cell lysis DNA extraction polymerase chain reaction(PCR) |
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