| 沙塘鳢不同组织同工酶生化表现型及遗传基础 |
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| 引用本文： | 殷文莉,戴建华. 沙塘鳢不同组织同工酶生化表现型及遗传基础[J]. 湖北师范学院学报(自然科学版), 2002, 22(4): 10-13 |
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| 作者姓名： | 殷文莉 戴建华 |
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| 作者单位： | 湖北师范学院,生物系,湖北,黄石,435002 |
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| 基金项目： | 湖北省自然科学基金资助(97J049) |
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| 摘 要： | 采用垂直板聚丙烯酰胺凝胶电泳方法，对沙塘鳢（Odontobutis abscura）的消化道、心脏、肌肉、脾脏、肾脏、脑、肝脏、生殖腺和晶体9种组织的4种同工酶(EST、LDH、ADH、MDH)进行了研究，讨论了这几种同工酶在沙塘鳢不同组织中的分布以及遗传基础。EST有5个基因位点，单体酶；LDH只有1个基因位点，为四聚体酶；MDH为由3个基因位点控制的二聚体酶；ADH亦为二聚体酶，但仅在肝脏中得到表达。结果表明，同工酶的表达具有组织特异性。
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| 关 键 词： | 沙塘鳢 同工酶 生化表现型 遗传基础 基因位点 组织特异性 基因表达 |
| 文章编号： | 1009-2714(2002)04-0010-04 |
| 修稿时间： | 2002-06-28 |
The phenotypes and genetic basis of isozymes from different tissues of Odontobutis obscura |
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| YIN Wen-li DAI Jian-hua. The phenotypes and genetic basis of isozymes from different tissues of Odontobutis obscura[J]. Journal of Hubei Normal University(Natural Science), 2002, 22(4): 10-13 |
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| Authors: | YIN Wen-li DAI Jian-hua |
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| Abstract: | By using the vertical polyacrylamide gel electrophoresis, 4 isozymes (LDH, EST, MDH, ADH) in digestive tract, heart, muscle, spleen, kidney, brain, liver, sexual gland and eye of Odontobutis obscura were studied and the phenotypes and genetic basis of the 4 isozymes were analysed. EST had 5 loci and 2 alleles; LDH, 1 locus and 2 alleles; MDH, 3 loci and 2 alleles; ADH, 1 locus and 1 allele, respectively. The results indicated that isozyme genes exhibited a tissue specific expression. |
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| Keywords: | Odontobutis obscura isozymes phenotypes genetic basis |
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