| 乳酸菌的分离和鉴定及其1,3-PDO的活性测定 |
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| 引用本文： | 张静,白林含,李世林,黄非,徐颖,李茜,乔代蓉,曹毅.乳酸菌的分离和鉴定及其1,3-PDO的活性测定[J].四川大学学报(自然科学版),2006,43(3):672-676. |
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| 作者姓名： | 张静 白林含 李世林 黄非 徐颖 李茜 乔代蓉 曹毅 |
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| 作者单位： | 四川大学生命科学学院,四川省生物信息与代谢工程共享实验平台,四川大学生物资源与生态环境教育部重点实验室,成都,610065 |
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| 基金项目： | 四川省科技厅重点项目(04NG002-006-1) |
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| 摘 要： | 从红原取样的酸奶中分离出7株乳酸菌,根据《伯杰氏细菌鉴定手册》和《乳酸细菌分类鉴定及实验方法》,依据细菌的菌落特征、菌体形态、生理生化特性初步鉴定这7株乳酸菌分别属于乳球菌属,链球菌属,乳杆菌属.并且通过1,3-丙二醇氧化还原酶活性检测,发现其中一株具有活性.
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| 关 键 词： | 乳酸菌 分离 鉴定 1 3-丙二醇 3-丙二醇氧化还原酶 |
| 文章编号： | 0490-6756（2006）03-0672-05 |
| 收稿时间： | 2005-10-18 |
| 修稿时间： | 2005-10-18 |
Isolation,Identification of Several Lactic Acid Bacteria and Their Enzyme Assays of 1,3-PDO |
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| ZHANG Jing,BAI Lin-han,LI Shi-lin,HUANG Fei,XU Ying,LI Qian,QIAO Dai-rong,CAO Yi.Isolation,Identification of Several Lactic Acid Bacteria and Their Enzyme Assays of 1,3-PDO[J].Journal of Sichuan University (Natural Science Edition),2006,43(3):672-676. |
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| Authors: | ZHANG Jing BAI Lin-han LI Shi-lin HUANG Fei XU Ying LI Qian QIAO Dai-rong CAO Yi |
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| Institution: | College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education |
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| Abstract: | in this research, seven Lactic acid bacteria were isolated from the yoghourt which was sampled from Hongyuan. The characteristic and biochemical analysis showed that the seven LAB belonged to Lactobacillus, Lactococcusand and Streptococcus. Additionally, one of the LAB was found to have the 1,3-PDO activity through the enzyme assays of 1,3-PDO. |
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| Keywords: | Lactic acid bacteria(LAB) isolation identification 1 3-propanediol 1 3-PDL Oxidoreductases |
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