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| 脂肪酶催化制备丁酸乙酯的研究 | |
| 引用本文： | 沈咏梅,谢晓娜,荀二娜,王佳欣,张弘,王磊,王智.脂肪酶催化制备丁酸乙酯的研究[J].成都大学学报(自然科学版),2012,31(1):4-7. |
| 作者姓名： | 沈咏梅 谢晓娜 荀二娜 王佳欣 张弘 王磊 王智 |
| 作者单位： | 1. 吉林大学白求恩医科大学制药厂,吉林长春130021／吉林大学分子酶学工程教育部重点实验室,吉林长春130023 2. 吉林大学第一临床医院,吉林长春,130021 3. 吉林大学分子酶学工程教育部重点实验室,吉林长春,130023 |
| 基金项目： | 国家自然科学基金（31070708,20803028,30870539）;吉林大学科学前沿与交叉学科创新项目（200903099）资助项目. |
| 摘 要： | 利用脂肪酶BSL2作为催化剂,在有机介质中催化合成丁酸乙酯.考察了反应温度、底物浓度、底物摩尔比、反应介质和初始水活度等反应条件对酶促合成丁酸乙酯的影响.试验结果表明:以正己烷为溶剂,初始水活度为0.33,加酶量为100 mg,丁酸浓度为40 mM,底物摩尔比(丁酸/乙醇)为1∶1.5,在40℃条件下振荡反应30 h,合成丁酸乙酯的的转化率可达到94.8%. |
| 关 键 词： | 丁酸乙酯 脂肪酶 有机介质 酯合成反应速度 |
Research on Preparation of Ethyl Butyrate Catalyzed by Lipase |
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| SHEN Yongmei,XIE Xiaona,XUN Erna,WANG Jiaxin,ZHANG Hong,WANG Lei,WANG Zhi.Research on Preparation of Ethyl Butyrate Catalyzed by Lipase[J].Journal of Chengdu University (Natural Science),2012,31(1):4-7. | |
| Authors: | SHEN Yongmei XIE Xiaona XUN Erna WANG Jiaxin ZHANG Hong WANG Lei WANG Zhi |
| Institution: | 1 .Pharmaceutical Factory of Norman Bethune University of Medical Sciences, Jilin University, Cllangchun 130021, China; 2. The First Hospital of Jilin University, Jilin University, Changchun 130023, China; 3. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, C＇hangchun 130021, China) |
| Abstract: | Bacillus subtilis lipase（BSL2） was used to catalyze the synthesis of ethyl butyrate in organic sol- vents. The effects of reaction conditions such as reaction temperature, concentration of substrate, molar ratio of substrate, reaction medium, initial water activity and others on the synthesis of ethyl butyrate catalyzed by lipase were investigated to obtain the optimum reaction conditions. The findings show that percent conver- sion of synthesis of ethyl butyrate reaches 94.8% under the optimum conditions of using n-hexane as solu- tion,0.33 of initial water activity,with 100 mg lipase and 40 mM concentration of butyric acid, 1 ： 1.5 of molar ratio of substrate, at the temperature of 40℃ after 30 h oscillatory reaction. |
| Keywords: | ethyl butyrate lipase organic medium percent conversion of synthesis of ethyl butyrate |
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