基于流式细胞术和K-mer方法测定6种槭属植物基因组大小

【目的】槭属植物种类繁多，具有观赏、油用、药用等重要价值，开发应用前景广阔。基于流式细胞术和K-mer分析方法，首次进行6种槭树基因组大小测定，为槭属植物分子生物学、细胞学研究提供重要理论参考。【方法】以6种槭树嫩叶为材料，WPB为细胞核解离液，大豆(Glycine max‘Polanka’)和玉米(Zea maysCE-777)为内标，利用流式细胞检测技术和K-mer分析法估测6种槭树的基因组大小。【结果】基于流式细胞术测定建始槭(Acer henryi)、三角枫(A.buergerianum)、秀丽槭(A.elegantulum)、血皮槭(A.griseum)、五小叶槭(A.pentaphyllum)和青楷槭(A.tegmentosum)的基因组大小分别为(691.12±8.69)、(863.90±8.69)、(896.50±4.35)、(893.24±8.69)、(766.10±8.69)和(1 154.04±13.04) Mb;以K-mer分析法估测的基因组大小分别为561.72、743.00、777.87、771.51、650.64和1 103.46 Mb;杂合率分别为1.0...

Estimation of genome sizes of six Acer species by flow cytometry and K-mer analysis

【Objective】 There are variety species in the genus Acer, which have broad prospects for development and application in the ornamental, oil and medicinal fields. To provide an important theoretical reference for molecular biology and cytology studies of Acer, the genome sizes of six species of Acer were estimated using flow cytometry and K-mer analysis. 【Method】 Fresh leaves of six species of Acer were collected, and woody plant buffer(WPB) was used as a nuclear dissociation solution, Glycine max ‘Polanka’ and Zea mays CE-777 as internal standard. Six genome sizes were estimated by flow cytometry and K-mer analysis. 【Result】 The results of flow cytometry showed that the genomes of Acer henryi, A. buergerianum, A. elegantulum, A. griseum, A. pentaphyllum and A. tegmentosum were successfully estimated. The genome sizes of those were (691.12±8.69),(863.90±8.69), (896.50±4.35), (893.24±8.69), (766.10±8.69) and (1 154.04±13.04) Mb, respectively. The K-mer analysis showed that they were 561.72, 743.00, 777.87, 771.51, 650.64 and 1 103.46 Mb, respectively. The hybridity rate was about 1.00%、1.24%、1.31%、1.08%、0.28% and 0.81%, respectively,which were slightly smaller than that flow cytometry. 【Conclusion】 The results of this study could enrich the Acer gene database and provide important foundations for genomic and molecular cytogenetics studies within this genus.