The implantation of sepharose beads in mouse livers as an aid in the study of hepatic schistosomal fibrosis |
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| Authors: | E A E van Marck S Stocker J A Grimaud L Kestens P L J Gigase A M Deelder |
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| Institution: | (1) Department of Histopathology and Experimental Schistosomiasis, Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, (Belgium);(2) Centre de Microscopie Electronique et de Pathologie Ultrastructurale and Laboratoire de Pathologie Cellulaire du Foie, C.N.R.S., ERA 819, Institut Pasteur, 77, rue Pasteur, F-69365 Lyon, (France);(3) Medical Faculty, Laboratory of Parasitology, Leiden University, Rapenburg 33, Leiden, (The Netherlands) |
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| Abstract: | Summary An experimental model of schistosomal portal fibrosis is described. Sepharose beads the size of schistosome eggs, loaded or not with soluble egg antigen (SEA) fromSchistosoma mansoni, are injected into the coecal vein of C3H/Sn mice and become embolized in the liver. Only SEA-coated beads evoke a granulomatous reaction; this is enhanced by simultaneous priming of the mice with spleen cells fromSchistosoma mansoni-infected syngeneic animals. The fibrosis, which ensues around the beads, is stable and is much more evident after priming. Preliminary collagen tissue immunotyping reveals the presence of collagen deposits of types I and III collagen. Type IV collagen remains unchanged in the portal tracts. The model appears to be well suited for studies of the pathogenesis of portal fibrosis.This work was supported by a contract from INSERM (Action Spéciale No 3). |
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