| 抗汉坦病毒单链抗体双元载体的构建 |
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| 引用本文： | 罗雯 张九东 吴兴安 白文涛. 抗汉坦病毒单链抗体双元载体的构建[J]. 科学技术与工程, 2004, 4(11): 921-924 |
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| 作者姓名： | 罗雯 张九东 吴兴安 白文涛 |
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| 作者单位： | 西安文理学院化学与生命科学系,西安,710065;第四军医大学微生物学教研室,西安,710032 |
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| 基金项目： | 西安市科技局社会与发展计划(200345)资助 |
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| 摘 要： | 利用PCR方法，从含有1A8 scFv基因的重组质粒中扩增出抗体基因，并使基因两端携带合适的限制性酶切位点。经多步连接将其克隆入植物表达载体pBI121或pCAMBIA1305．2。酶切结果证明1A8 scFv基因被成功克隆入植物表达载体pBI121及pCAMBIA1305．2，构建获得1A8 scFv-p BI121及1A8 scFv pCAMBI A1305．2重组质粒，并将其转入农杆菌GV3101。抗汉坦病毒mAb 1A 8scFv植物双元表达载体的获得，为进一步在植物中表达该抗体片段奠定了基础。
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| 关 键 词： | 汉坦病毒 单链抗体 植物表达载体 |
| 文章编号： | 1671-1815(2004)11-0921-04 |
| 修稿时间： | 2004-06-23 |
Construction of Plant Expression Vectors Containing Single Chain Fv Gene Fragment Against Hantavirus |
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| O Wen,ZHANG Jiudong,WU Xing''an,BAI Wentao. Construction of Plant Expression Vectors Containing Single Chain Fv Gene Fragment Against Hantavirus[J]. Science Technology and Engineering, 2004, 4(11): 921-924 |
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| Authors: | O Wen ZHANG Jiudong WU Xing''an BAI Wentao |
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| Abstract: | 8 scFv gene fragment was amplified and proper restriction enzyme sites were added at each end by PCR. It was cloned into pBI121 or pCAMBIA1305. 2 through several cloning steps. The recombi-nants 1A8 scFv-pBI121 and 1A8 scFv-pCAMBIA1305. 2 were proved to be constructed successfully by restriction enzyme analysis and further transformed into GV3101. The construction of the recombinant plant expression vectors containing the scFv gene fragment of monoclonal antibody 1A8 against Hantavirus has laid a solid foundation for further study on expression of certain antibody fragment in plant. |
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| Keywords: | ntavirus single chain Fv plant expression vector |
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