大黄牡丹汤组方对急性胰腺炎模型大鼠腺泡细胞 分泌功能的影响

摘要: 目的 研究大黄牡丹汤组方( RPDP) 对急性胰腺炎( Acute pancreatitis，AP) 模型大鼠 胰腺腺泡细胞外分泌功 能和腺泡细胞内钙离子浓度( FI) 的影响。方法 SD 大鼠灌胃 RPDP 以制备大黄牡丹汤组方含药血清( RPDP-S) ; SD 大鼠分为假手术组和 AP 模型组，分离胰腺腺泡细胞并与 RPDP-S 共同孵育，测定腺泡细胞淀粉酶分泌水平和 FI 变化。结果 AP 模型大鼠腺泡细胞分泌淀粉酶水平较假手术组显 著升高( P ＜ 0. 05) ，经 RPDP-S 处理的 AP 模 型大鼠腺泡细胞分泌淀粉酶水平显著降低( P ＜ 0. 05) ; FI 随 AP 模型病程延长而升高( P ＜ 0. 05) ，RPDP-S 可抑制 AP 模型大鼠腺泡细胞内 FI 升高( P ＜ 0. 05) 。结论 ＲPDP 通能抑制 AP 大鼠胰腺腺泡细胞的外分泌功能，抑制腺 泡细胞内钙离子的升高，降低腺泡细胞内 FI 超载。

The Effect of Dahuang Mudan Decoction on Exocrine Function of Pancreatic Acinar Cells in Acute Pancreatitis Model Rats

Abstract: Objective To study the effect of Dahuang Mudan Decoction ( ＲPDP) on exocrine function and intracellular Ca2 + concentration( FI) of pancreatic acinar cells in acute pancreatitis ( AP) model rats． Method Dahuang Mudan Decoction was administrated to SD rats and the serum containing Dahuang Mudan Decoction ( ＲPDP-S) wasprepared． SD rats randomly divided into three groups: the normal control group，the sham operation ( SO) group and the AP group． Separate pancreatic acinar cells were isolated and co-incubated with ＲPDP-S， amylase secretion of pancreatic acinar cells and the change of FI were analyzed． Ｒesult Compared with the SO group，amylase secretion of pancreatic acinar cells was significantly increase in AP group( P ＜ 0. 05) ; and amylase secretion was significantly decrease in the pancreatic acinar cells treated with ＲPDP-S( P ＜ 0. 05) ，and the FI was raise up along with the prolonged disease ( P ＜ 0. 05 ) ． Conclusion ＲPDP can decrease intracellular Ca2 + concentration，and inhibit the overload of FIand the exocrine function of pancreatic acinar cells．