高盐高蛋白饮食对 Dah1 / SS 大鼠血压、肾脏和消化道 病理变化的影响

摘要: 目的 探讨高盐高蛋白饮食对 Dah1 / SS 大鼠血压、肾脏和消化道病理变化的影响。方法 将 30 只 SPF 级雄性 Dah1 / SS 大鼠随机分为 3 个组，即对照组、高盐组和高蛋白组，分别给予 0. 4% NaCl 普通低盐饲料、8% NaCl 高盐饲 料和 2. 4% 蛋氨酸饲料，喂养 2 个月，每周测量体质量及收缩压 1 次。实验结束，取大鼠肾脏、食管、胃、小肠、直肠 及胰腺经 HE 染色后进行病理学观察。结果 高盐、高蛋白组大鼠体质量增长明显低于对照组; 高盐组大鼠收缩压 高于对照组和高蛋白组大鼠，差异有统计学意义，高蛋白组大鼠未见血压升高; 高盐及高蛋白组大鼠出现了不同程 度的肾功能损伤，高盐组大鼠出现了肾脏病理性损伤; 高盐和高蛋白饮食未见消化道及胰腺损伤。结论 Dah1 / SS 大鼠高盐饮食可成功建立盐敏感性高血压模型，且高盐高蛋白饮食对肾脏会造成不同程度的损伤，而对消化道及 胰腺未造成损伤。

The Effect of High Salt and High Protein Diet on Blood Pressure and the Pathological Changes of Kidney and Gastrointestinal in Dah1 /SS Rats

Abstract: Objective To explore the effect of high salt and high protein diet on blood pressure and the pathology changes of kidney and gastrointestinal in Dah1 / SS rats． Method SPF female Dah1 / SS rats were respectively randomly divided into three groups: control，high-salt and high-protein group，and were respectively fed to common low-salt diet，high-salt diet witch contain 8% sodium chloride and high-protein diet with 2. 4% methionine in the individual ventilated cages ( IVC) ． Systolic blood pressure ( SBP) and body weight of rats was measured every week． At 8 weeks，the kidney，esophagus，stomach ，small intestine ，rectum，and pancreas tissues were stained with HE to observe the pathological changes． Result The rat body weight of high-salt and high-protein group were significantly lower than the control group，but the SBP of high-salt group was significantly higher than control and high-protein group． Renal impairment was observed in high-salt and high-protein group． Besides，the pathological changes of kidney were observe only in high-salt group． Also，the pathological changes of esophagus，stomach， small intestine，rectum，and pancreas tissues were not found． Conclusion The salt-sensitive hypertension model was successfully made by Dah1 / SS rats fed with high salt diet，and the varying degrees of damage of kidney with high salt and high protein diet，but no damage to gastrointestinal and pancreas．